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10.1038/srep11405 http://scihub22266oqcxt.onion/10.1038/srep11405 C4473686!4473686!26091540     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2015 ; 5 (ä): ä Nephropedia Template TP {{tp|p=26091540|t=2015. Evolution of an Enzyme from a Noncatalytic Nucleic Acid Sequence |pdf=|usr=}}{{26091540}} gab.com Text Evolution of an Enzyme from a Noncatalytic Nucleic Acid Sequence JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26091540 #FOAvip The mechanism by which enzymes arose from both abiotic and biological worlds remains an unsolved natural mystery. We postulate that an enzyme can emerge from any sequence of any functional polymer under permissive evolutionary conditions. To support this premise, we have arbitrarily chosen a 50-nucleotide DNA fragment encoding for the Bos taurus (cattle) albumin mRNA and subjected it to test-tube evolution to derive a catalytic DNA (DNAzyme) with RNA-cleavage activity. After only a few weeks, a DNAzyme with significant catalytic activity has surfaced. Sequence comparison reveals that seven nucleotides are responsible for the conversion of the noncatalytic sequence into the enzyme. Deep sequencing analysis of DNA pools along the evolution trajectory has identified individual mutations as the progressive drivers of the molecular evolution. Our findings demonstrate that an enzyme can indeed arise from a sequence of a functional polymer via permissive molecular evolution, a mechanism that may have been exploited by nature for the creation of the enormous repertoire of enzymes in the biological world today. Twit Text FOAVip Evolution of an Enzyme from a Noncatalytic Nucleic Acid Sequence ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26091540 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26091540 #FOAvip English Wikipedia <ref>{{Cite pmid|26091540}}</ref> Evolution of an Enzyme from a Noncatalytic Nucleic Acid Sequence #MMPMID26091540 Gysbers R; Tram K; Gu J; Li Y Sci Rep 2015[]; 5 (ä): ä PMID26091540show ga The mechanism by which enzymes arose from both abiotic and biological worlds remains an unsolved natural mystery. We postulate that an enzyme can emerge from any sequence of any functional polymer under permissive evolutionary conditions. To support this premise, we have arbitrarily chosen a 50-nucleotide DNA fragment encoding for the Bos taurus (cattle) albumin mRNA and subjected it to test-tube evolution to derive a catalytic DNA (DNAzyme) with RNA-cleavage activity. After only a few weeks, a DNAzyme with significant catalytic activity has surfaced. Sequence comparison reveals that seven nucleotides are responsible for the conversion of the noncatalytic sequence into the enzyme. Deep sequencing analysis of DNA pools along the evolution trajectory has identified individual mutations as the progressive drivers of the molecular evolution. Our findings demonstrate that an enzyme can indeed arise from a sequence of a functional polymer via permissive molecular evolution, a mechanism that may have been exploited by nature for the creation of the enormous repertoire of enzymes in the biological world today. äEvolution of an Enzyme from a Noncatalytic Nucleic Acid Sequence (epmc).pdf DeepDyve Pubget Overpricing