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10.5114/ceji.2015.50837 http://scihub22266oqcxt.onion/10.5114/ceji.2015.50837 C4472543!4472543!26155187     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cent+Eur+J+Immunol 2015 ; 40 (1): 78-82 Nephropedia Template TP {{tp|p=26155187|t=2015. Prohibitin as a novel autoantigen in rheumatoid arthritis |pdf=|usr=}}{{26155187}} gab.com Text Prohibitin as a novel autoantigen in rheumatoid arthritis JO: Cent Eur J Immunol http://www.kidney.de/pget.php?&tw=1&pmid=26155187 #FOAvip The aim of this study: The aim of this study was to verify whether prohibitin is a novel autoantigen in rheumatoid arthritis. Material and methods: First, recombinant human prohibitin (rhPHB) protein was cloned, expressed, and purified. Then the anti-prohibitin autoantibodies were detected by western blotting by using rhPHB protein to incubate sera from patients with rheumatoid arthritis (RA). Next, immunoprecipitation was employed to further illustrate whether anti-prohibitin antibodies exist in RA patients. And finally, autoantibodies against the rhPHB protein were investigated using a homemade ELISA kit through the assessment of 258 real clinical samples. Results: It was revealed that anti-prohibitin antibodies existed in the sera of patients with RA. Reactivity of serum IgG against rhPHB was detected in 26 of 86 RA patients (30.3%), 7 of 86 systemic lupus erythematosus (SLE) patients (8.1%), and 1 of 86 apparently healthy donors (HC) (1.2%). Conclusions: Prohibitin was proved to be a novel autoantigen and the corresponding anti-prohibitin autoantibodies were present in the RA patients' blood circulation. Twit Text FOAVip Prohibitin as a novel autoantigen in rheumatoid arthritis ????Cent Eur J Immunol http://www.kidney.de/pget.php?&tw=1&pmid=26155187 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26155187 #FOAvip English Wikipedia <ref>{{Cite pmid|26155187}}</ref> Prohibitin as a novel autoantigen in rheumatoid arthritis #MMPMID26155187 Shi LL; Chen P; Xun YP; Yang WK; Yang CH; Chen GY; Du HW Cent Eur J Immunol 2015[]; 40 (1): 78-82 PMID26155187show ga The aim of this study: The aim of this study was to verify whether prohibitin is a novel autoantigen in rheumatoid arthritis. Material and methods: First, recombinant human prohibitin (rhPHB) protein was cloned, expressed, and purified. Then the anti-prohibitin autoantibodies were detected by western blotting by using rhPHB protein to incubate sera from patients with rheumatoid arthritis (RA). Next, immunoprecipitation was employed to further illustrate whether anti-prohibitin antibodies exist in RA patients. And finally, autoantibodies against the rhPHB protein were investigated using a homemade ELISA kit through the assessment of 258 real clinical samples. Results: It was revealed that anti-prohibitin antibodies existed in the sera of patients with RA. Reactivity of serum IgG against rhPHB was detected in 26 of 86 RA patients (30.3%), 7 of 86 systemic lupus erythematosus (SLE) patients (8.1%), and 1 of 86 apparently healthy donors (HC) (1.2%). Conclusions: Prohibitin was proved to be a novel autoantigen and the corresponding anti-prohibitin autoantibodies were present in the RA patients' blood circulation. äProhibitin as a novel autoantigen in rheumatoid arthritis (epmc).pdf DeepDyve Pubget Overpricing