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10.1186/s12858-015-0044-7 http://scihub22266oqcxt.onion/10.1186/s12858-015-0044-7 C4471906!4471906!26087769     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       BMC+Biochem 2015 ; 16 (ä): ä Nephropedia Template TP {{tp|p=26087769|t=2015. Human POLD1 modulates cell cycle progression and DNA damage repair |pdf=|usr=}}{{26087769}} gab.com Text Human POLD1 modulates cell cycle progression and DNA damage repair JO: BMC Biochem http://www.kidney.de/pget.php?&tw=1&pmid=26087769 #FOAvip Background: The activity of eukaryotic DNA polymerase delta (Pol ?) plays an essential role in genome stability through its effects on DNA replication and repair. The p125 catalytic subunit of Pol ? is encoded by POLD1 gene in human cells. To clarify biological functions of POLD1, we investigated the effects of POLD1 overexpression or downregulation on cell proliferation, cell cycle progression, DNA synthesis and oxidative DNA damage induced by H2O2. Methods: HEK293 cells were transfected with POLD1 expression plasmid or shRNA, cell proliferation, cell cycle progression, and DNA synthesis in HEK293 cells were analyzed. Results: HEK293 cells were transfected with POLD1 expression plasmid or shRNA. POLD1 downregulation by shRNA suppressed cell proliferation, cell cycle progression, and DNA synthesis in HEK293 cells. However, POLD1 overexpression had no significant effects on these processes. Finally, comet assay showed that POLD1 downregulation led to increased DNA damage. Conclusions: Our results suggest that human POLD1 plays important role in the regulation of cell cycle progression and DNA damage repair. Twit Text FOAVip Human POLD1 modulates cell cycle progression and DNA damage repair ????BMC Biochem http://www.kidney.de/pget.php?&tw=1&pmid=26087769 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26087769 #FOAvip English Wikipedia <ref>{{Cite pmid|26087769}}</ref> Human POLD1 modulates cell cycle progression and DNA damage repair #MMPMID26087769 Song J; Hong P; Liu C; Zhang Y; Wang J; Wang P BMC Biochem 2015[]; 16 (ä): ä PMID26087769show ga Background: The activity of eukaryotic DNA polymerase delta (Pol ?) plays an essential role in genome stability through its effects on DNA replication and repair. The p125 catalytic subunit of Pol ? is encoded by POLD1 gene in human cells. To clarify biological functions of POLD1, we investigated the effects of POLD1 overexpression or downregulation on cell proliferation, cell cycle progression, DNA synthesis and oxidative DNA damage induced by H2O2. Methods: HEK293 cells were transfected with POLD1 expression plasmid or shRNA, cell proliferation, cell cycle progression, and DNA synthesis in HEK293 cells were analyzed. Results: HEK293 cells were transfected with POLD1 expression plasmid or shRNA. POLD1 downregulation by shRNA suppressed cell proliferation, cell cycle progression, and DNA synthesis in HEK293 cells. However, POLD1 overexpression had no significant effects on these processes. Finally, comet assay showed that POLD1 downregulation led to increased DNA damage. Conclusions: Our results suggest that human POLD1 plays important role in the regulation of cell cycle progression and DNA damage repair. äHuman POLD1 modulates cell cycle progression and DNA damage repair (epmc).pdf DeepDyve Pubget Overpricing