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10.1016/j.stemcr.2015.04.016 http://scihub22266oqcxt.onion/10.1016/j.stemcr.2015.04.016 C4471821!4471821!26028531     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26028531&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Stem+Cell+Reports 2015 ; 4 (6): 1103-11 Nephropedia Template TP {{tp|p=26028531|t=2015. A CRISPR/Cas-Mediated Selection-free Knockin Strategy in Human Embryonic Stem Cells |pdf=|usr=}}{{26028531}} gab.com Text A CRISPR/Cas-Mediated Selection-free Knockin Strategy in Human Embryonic Stem Cells JO: Stem Cell Reports http://www.kidney.de/pget.php?&tw=1&pmid=26028531 #FOAvip The development of new gene-editing tools, in particular the CRISPR/Cas system, has greatly facilitated site-specific mutagenesis in human embryonic stem cells (hESCs), including the introduction or correction of patient-specific mutations for disease modeling. However, integration of a reporter gene into an endogenous locus in hESCs still requires a lengthy and laborious two-step strategy that involves first drug selection to identify correctly targeted clones and then excision of the drug-resistance cassette. Through the use of iCRISPR, an efficient gene-editing platform we recently developed, this study demonstrates a knockin strategy without drug selection for both active and silent genes in hESCs. Lineage-specific hESC reporter lines are useful for real-time monitoring of cell-fate decisions and lineage tracing, as well as enrichment of specific cell populations during hESC differentiation. Thus, this selection-free knockin strategy is expected to greatly facilitate the use of hESCs for developmental studies, disease modeling, and cell-replacement therapy. Twit Text FOAVip A CRISPR/Cas-Mediated Selection-free Knockin Strategy in Human Embryonic Stem Cells ????Stem Cell Reports http://www.kidney.de/pget.php?&tw=1&pmid=26028531 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26028531 #FOAvip English Wikipedia <ref>{{Cite pmid|26028531}}</ref> A CRISPR/Cas-Mediated Selection-free Knockin Strategy in Human Embryonic Stem Cells #MMPMID26028531 Zhu Z; Verma N; González F; Shi ZD; Huangfu D Stem Cell Reports 2015[Jun]; 4 (6): 1103-11 PMID26028531show ga The development of new gene-editing tools, in particular the CRISPR/Cas system, has greatly facilitated site-specific mutagenesis in human embryonic stem cells (hESCs), including the introduction or correction of patient-specific mutations for disease modeling. However, integration of a reporter gene into an endogenous locus in hESCs still requires a lengthy and laborious two-step strategy that involves first drug selection to identify correctly targeted clones and then excision of the drug-resistance cassette. Through the use of iCRISPR, an efficient gene-editing platform we recently developed, this study demonstrates a knockin strategy without drug selection for both active and silent genes in hESCs. Lineage-specific hESC reporter lines are useful for real-time monitoring of cell-fate decisions and lineage tracing, as well as enrichment of specific cell populations during hESC differentiation. Thus, this selection-free knockin strategy is expected to greatly facilitate the use of hESCs for developmental studies, disease modeling, and cell-replacement therapy. äA CRISPR/Cas-Mediated Selection-free Knockin Strategy in Human Embryon(epmc).pdf DeepDyve Pubget Overpricing