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10.1007/s00216-015-8585-6 http://scihub22266oqcxt.onion/10.1007/s00216-015-8585-6 C4471391!4471391!25749796     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Anal+Bioanal+Chem 2015 ; 407 (17): 5189-98 Nephropedia Template TP {{tp|p=25749796|t=2015. Rapid evaluation of 25 key sphingolipids and phosphosphingolipids in human plasma by LC-MS/MS |pdf=|usr=}}{{25749796}} gab.com Text Rapid evaluation of 25 key sphingolipids and phosphosphingolipids in human plasma by LC-MS/MS JO: Anal Bioanal Chem http://www.kidney.de/pget.php?&tw=1&pmid=25749796 #FOAvip We report on a new, sensitive, and fast LC-MS/MS method for the simultaneous determination of 25 key sphingolipid components in human plasma, including phosphorylated sphinganine and sphingosine, in a single 9-min run. This method enables an effective and high-throughput coverage of the metabolic changes involving the sphingolipidome during physiological or pathological states. The method is based on liquid?liquid extraction followed by reversed-phase LC-MS/MS. Exogenous odd-chain lipids are used as cost-effective but reliable internal standards. The method was fully validated in surrogate matrix and naive human plasma following FDA guidelines. Sample stability and dilution integrity were also tested and verified.Electronic supplementary material: The online version of this article (doi:10.1007/s00216-015-8585-6) contains supplementary material, which is available to authorized users. Twit Text FOAVip Rapid evaluation of 25 key sphingolipids and phosphosphingolipids in human plasma by LC-MS/MS ????Anal Bioanal Chem http://www.kidney.de/pget.php?&tw=1&pmid=25749796 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25749796 #FOAvip English Wikipedia <ref>{{Cite pmid|25749796}}</ref> Rapid evaluation of 25 key sphingolipids and phosphosphingolipids in human plasma by LC-MS/MS #MMPMID25749796 Basit A; Piomelli D; Armirotti A Anal Bioanal Chem 2015[]; 407 (17): 5189-98 PMID25749796show ga We report on a new, sensitive, and fast LC-MS/MS method for the simultaneous determination of 25 key sphingolipid components in human plasma, including phosphorylated sphinganine and sphingosine, in a single 9-min run. This method enables an effective and high-throughput coverage of the metabolic changes involving the sphingolipidome during physiological or pathological states. The method is based on liquid?liquid extraction followed by reversed-phase LC-MS/MS. Exogenous odd-chain lipids are used as cost-effective but reliable internal standards. The method was fully validated in surrogate matrix and naive human plasma following FDA guidelines. Sample stability and dilution integrity were also tested and verified.Electronic supplementary material: The online version of this article (doi:10.1007/s00216-015-8585-6) contains supplementary material, which is available to authorized users. äRapid evaluation of 25 key sphingolipids and phosphosphingolipids in h(epmc).pdf DeepDyve Pubget Overpricing