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10.1016/j.hrthm.2012.10.002 http://scihub22266oqcxt.onion/10.1016/j.hrthm.2012.10.002 C4470703!4470703!23041576     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Heart+Rhythm 2013 ; 10 (1): 80-7 Nephropedia Template TP {{tp|p=23041576|t=2013. The Ionic Bases of the Action Potential in Isolated Mouse Cardiac Purkinje Cell |pdf=|usr=}}{{23041576}} gab.com Text The Ionic Bases of the Action Potential in Isolated Mouse Cardiac Purkinje Cell JO: Heart Rhythm http://www.kidney.de/pget.php?&tw=1&pmid=23041576 #FOAvip Aims: Collecting electrophysiological and molecular data from the murine conduction system presents technical challenges. We have developed an approach for the isolation of murine Purkinje cells (PCs), characterized the major ionic currents and use the ionic data to simulate action potentials (APs) recorded from the isolated PCs. Methods and Results: Light microscopy was used to isolate and identify PCs from apical and septal cells. Current and voltage clamp techniques were used to record APs and whole cell currents. We simulated a PC action potential, based on our experimental data. APs recorded from PCs were significantly longer than those recorded from ventricular cells. The prominent plateau phase of the PC AP was very negative (~?40mV). Spontaneous activity was observed only in PCs. The inward rectifier current, IK1, demonstrated no significant differences compared to ventricular myocytes (VMs). However, sodium current density was larger, and the voltage-gated potassium current (Ito) density was significantly less in PCs compared to myocytes. T-Type Ca2+ currents (ICa-T) were present in PCs but not VMs. Computer simulations suggest that ICa-T and cytosolic calcium diffusion significantly modulate AP profile recorded in PCs, as compared to VMs. Conclusions: Our study provides the first comprehensive ionic profile of murine PCs. The data show unique features of PC ionic mechanisms that govern its excitation process. Experimental data and numerical modeling results suggest that a smaller Ito and the presence of the ICa-T are important determinants of the longer and relatively negative plateau phase of the APs. Twit Text FOAVip The Ionic Bases of the Action Potential in Isolated Mouse Cardiac Purkinje Cell ????Heart Rhythm http://www.kidney.de/pget.php?&tw=1&pmid=23041576 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=23041576 #FOAvip English Wikipedia <ref>{{Cite pmid|23041576}}</ref> The Ionic Bases of the Action Potential in Isolated Mouse Cardiac Purkinje Cell #MMPMID23041576 Vaidyanathan R; O?Connell RP; Deo M; Milstein ML; Furspan P; Herron TJ; Pandit SV; Musa H; Berenfeld O; Jalife J; Anumonwo JM Heart Rhythm 2013[Jan]; 10 (1): 80-7 PMID23041576show ga Aims: Collecting electrophysiological and molecular data from the murine conduction system presents technical challenges. We have developed an approach for the isolation of murine Purkinje cells (PCs), characterized the major ionic currents and use the ionic data to simulate action potentials (APs) recorded from the isolated PCs. Methods and Results: Light microscopy was used to isolate and identify PCs from apical and septal cells. Current and voltage clamp techniques were used to record APs and whole cell currents. We simulated a PC action potential, based on our experimental data. APs recorded from PCs were significantly longer than those recorded from ventricular cells. The prominent plateau phase of the PC AP was very negative (~?40mV). Spontaneous activity was observed only in PCs. The inward rectifier current, IK1, demonstrated no significant differences compared to ventricular myocytes (VMs). However, sodium current density was larger, and the voltage-gated potassium current (Ito) density was significantly less in PCs compared to myocytes. T-Type Ca2+ currents (ICa-T) were present in PCs but not VMs. Computer simulations suggest that ICa-T and cytosolic calcium diffusion significantly modulate AP profile recorded in PCs, as compared to VMs. Conclusions: Our study provides the first comprehensive ionic profile of murine PCs. The data show unique features of PC ionic mechanisms that govern its excitation process. Experimental data and numerical modeling results suggest that a smaller Ito and the presence of the ICa-T are important determinants of the longer and relatively negative plateau phase of the APs. äThe Ionic Bases of the Action Potential in Isolated Mouse Cardiac Purk(epmc).pdf DeepDyve Pubget Overpricing