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10.1038/mtm.2015.20 http://scihub22266oqcxt.onion/10.1038/mtm.2015.20 C4470365!4470365!26151065     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Ther+Methods+Clin+Dev 2015 ; 2 (ä): 15020- Nephropedia Template TP {{tp|p=26151065|t=2015. Large-scale production of lentiviral vector in a closed system hollow fiber bioreactor |pdf=|usr=}}{{26151065}} gab.com Text Large-scale production of lentiviral vector in a closed system hollow fiber bioreactor JO: Mol Ther Methods Clin Dev http://www.kidney.de/pget.php?&tw=1&pmid=26151065 #FOAvip Lentiviral vectors are widely used in the field of gene therapy as an effective method for permanent gene delivery. While current methods of producing small scale vector batches for research purposes depend largely on culture flasks, the emergence and popularity of lentiviral vectors in translational, preclinical and clinical research has demanded their production on a much larger scale, a task that can be difficult to manage with the numbers of producer cell culture flasks required for large volumes of vector. To generate a large scale, partially closed system method for the manufacturing of clinical grade lentiviral vector suitable for the generation of induced pluripotent stem cells (iPSCs), we developed a method employing a hollow fiber bioreactor traditionally used for cell expansion. We have demonstrated the growth, transfection, and vector-producing capability of 293T producer cells in this system. Vector particle RNA titers after subsequent vector concentration yielded values comparable to lentiviral iPSC induction vector batches produced using traditional culture methods in 225?cm2 flasks (T225s) and in 10-layer cell factories (CF10s), while yielding a volume nearly 145 times larger than the yield from a T225 flask and nearly three times larger than the yield from a CF10. Employing a closed system hollow fiber bioreactor for vector production offers the possibility of manufacturing large quantities of gene therapy vector while minimizing reagent usage, equipment footprint, and open system manipulation. Twit Text FOAVip Large-scale production of lentiviral vector in a closed system hollow fiber bioreactor ????Mol Ther Methods Clin Dev http://www.kidney.de/pget.php?&tw=1&pmid=26151065 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26151065 #FOAvip English Wikipedia <ref>{{Cite pmid|26151065}}</ref> Large-scale production of lentiviral vector in a closed system hollow fiber bioreactor #MMPMID26151065 Sheu J; Beltzer J; Fury B; Wilczek K; Tobin S; Falconer D; Nolta J; Bauer G Mol Ther Methods Clin Dev 2015[]; 2 (ä): 15020- PMID26151065show ga Lentiviral vectors are widely used in the field of gene therapy as an effective method for permanent gene delivery. While current methods of producing small scale vector batches for research purposes depend largely on culture flasks, the emergence and popularity of lentiviral vectors in translational, preclinical and clinical research has demanded their production on a much larger scale, a task that can be difficult to manage with the numbers of producer cell culture flasks required for large volumes of vector. To generate a large scale, partially closed system method for the manufacturing of clinical grade lentiviral vector suitable for the generation of induced pluripotent stem cells (iPSCs), we developed a method employing a hollow fiber bioreactor traditionally used for cell expansion. We have demonstrated the growth, transfection, and vector-producing capability of 293T producer cells in this system. Vector particle RNA titers after subsequent vector concentration yielded values comparable to lentiviral iPSC induction vector batches produced using traditional culture methods in 225?cm2 flasks (T225s) and in 10-layer cell factories (CF10s), while yielding a volume nearly 145 times larger than the yield from a T225 flask and nearly three times larger than the yield from a CF10. Employing a closed system hollow fiber bioreactor for vector production offers the possibility of manufacturing large quantities of gene therapy vector while minimizing reagent usage, equipment footprint, and open system manipulation. äLarge-scale production of lentiviral vector in a closed system hollow (epmc).pdf DeepDyve Pubget Overpricing