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10.14348/molcells.2015.0103 http://scihub22266oqcxt.onion/10.14348/molcells.2015.0103 C4469905!4469905!25985872     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Cells 2015 ; 38 (6): 475-81 Nephropedia Template TP {{tp|p=25985872|t=2015. Measuring and Reducing Off-Target Activities of Programmable Nucleases Including CRISPR-Cas9 |pdf=|usr=}}{{25985872}} gab.com Text Measuring and Reducing Off-Target Activities of Programmable Nucleases Including CRISPR-Cas9 JO: Mol Cells http://www.kidney.de/pget.php?&tw=1&pmid=25985872 #FOAvip Programmable nucleases, which include zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and RNA-guided engineered nucleases (RGENs) repurposed from the type II clustered, regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) system are now widely used for genome editing in higher eukaryotic cells and whole organisms, revolutionising almost every discipline in biological research, medicine, and biotechnology. All of these nucleases, however, induce off-target mutations at sites homologous in sequence with on-target sites, limiting their utility in many applications including gene or cell therapy. In this review, we compare methods for detecting nuclease off-target mutations. We also review methods for profiling genome-wide off-target effects and discuss how to reduce or avoid off-target mutations. Twit Text FOAVip Measuring and Reducing Off-Target Activities of Programmable Nucleases Including CRISPR-Cas9 ????Mol Cells http://www.kidney.de/pget.php?&tw=1&pmid=25985872 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25985872 #FOAvip English Wikipedia <ref>{{Cite pmid|25985872}}</ref> Measuring and Reducing Off-Target Activities of Programmable Nucleases Including CRISPR-Cas9 #MMPMID25985872 Koo T; Lee J; Kim JS Mol Cells 2015[Jun]; 38 (6): 475-81 PMID25985872show ga Programmable nucleases, which include zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and RNA-guided engineered nucleases (RGENs) repurposed from the type II clustered, regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) system are now widely used for genome editing in higher eukaryotic cells and whole organisms, revolutionising almost every discipline in biological research, medicine, and biotechnology. All of these nucleases, however, induce off-target mutations at sites homologous in sequence with on-target sites, limiting their utility in many applications including gene or cell therapy. In this review, we compare methods for detecting nuclease off-target mutations. We also review methods for profiling genome-wide off-target effects and discuss how to reduce or avoid off-target mutations. äMeasuring and Reducing Off-Target Activities of Programmable Nucleases(epmc).pdf DeepDyve Pubget Overpricing