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10.1117/1.2967983 http://scihub22266oqcxt.onion/10.1117/1.2967983 C4469470!4469470!19021327     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Biomed+Opt 2008 ; 13 (4): 041319 Nephropedia Template TP {{tp|p=19021327|t=2008. Extended cavity laser enhanced two-photon flow cytometry |pdf=|usr=}}{{19021327}} gab.com Text Extended cavity laser enhanced two-photon flow cytometry JO: J Biomed Opt http://www.kidney.de/pget.php?&tw=1&pmid=19021327 #FOAvip We demonstrate enhanced sensitivity in two-photon flow cytometry with an extended cavity laser excitation source. At low power, the home-built 20-MHz oscillator was able to detect a significantly larger fraction, in either phosphate buffered saline (PBS) or whole blood, of green fluorescent protein (GFP) ?expressing MCA-207 cells cross-labeled with the membrane-binding lipophilic dye DiD. A geometrical model is used to explain unique features of the signals resulting from the different spatial distribution of DiD and GFP. These unique features include sub-square law scaling of unsaturated two-photon signal, a sigmoidal sensitivity curve for detection under varying powers for cell detection thresholds as low as a single photon, and uncorrelated signal strengths in two detection channels. Twit Text FOAVip Extended cavity laser enhanced two-photon flow cytometry ????J Biomed Opt http://www.kidney.de/pget.php?&tw=1&pmid=19021327 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=19021327 #FOAvip English Wikipedia <ref>{{Cite pmid|19021327}}</ref> Extended cavity laser enhanced two-photon flow cytometry #MMPMID19021327 Tkaczyk ER; Tkaczyk AH; Katnik S; Ye JY; Luker KE; Luker GD; Myc A; Baker JR; Norris TB J Biomed Opt 2008[Jul]; 13 (4): 041319 PMID19021327show ga We demonstrate enhanced sensitivity in two-photon flow cytometry with an extended cavity laser excitation source. At low power, the home-built 20-MHz oscillator was able to detect a significantly larger fraction, in either phosphate buffered saline (PBS) or whole blood, of green fluorescent protein (GFP) ?expressing MCA-207 cells cross-labeled with the membrane-binding lipophilic dye DiD. A geometrical model is used to explain unique features of the signals resulting from the different spatial distribution of DiD and GFP. These unique features include sub-square law scaling of unsaturated two-photon signal, a sigmoidal sensitivity curve for detection under varying powers for cell detection thresholds as low as a single photon, and uncorrelated signal strengths in two detection channels. äExtended cavity laser enhanced two-photon flow cytometry (epmc).pdf DeepDyve Pubget Overpricing