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Rapid imaging of large tissues using high-resolution stage-scanning microscopy #MMPMID26137386
Yang T; Zheng T; Shang Z; Wang X; Lv X; Yuan J; Zeng S
Biomed Opt Express 2015[May]; 6 (5): 1867-75 PMID26137386show ga
Rapid and high-resolution imaging of large tissues is essential in biological research, like brain neuron connectivity research and cancer margins imaging. Here a novel stage-scanning confocal microscopy was developed for rapid imaging of large tissues. Line scanning methods and strip imaging strategy were used to increase the imaging speed. The scientific CMOS was used as line detector in sub-array mode and the optical sectioning ability can be easily adjusted by changing the number of line detectors according to different samples. Fluorescent beads imaging showed resolutions of 0.47 ?m, 0.56 ?m, and 1.56 ?m in the X, Y, and Z directions, respectively, with a 40 × objective lens. A 10 × 10 mm2 coronal plane with enough signal intensity could be imaged in about 88 sec at a sampling resolution of 0.16 ?m/pixel. Rapid imaging of mouse brain slices demonstrated the applicability of this system in visualizing neuronal details at high frame rate.