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10.1364/BOE.6.001767

http://scihub22266oqcxt.onion/10.1364/BOE.6.001767
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suck abstract from ncbi


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pmid26137379
      Biomed+Opt+Express 2015 ; 6 (5 ): 1767-81
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  • Molecular imaging needles: dual-modality optical coherence tomography and fluorescence imaging of labeled antibodies deep in tissue #MMPMID26137379
  • Scolaro L ; Lorenser D ; Madore WJ ; Kirk RW ; Kramer AS ; Yeoh GC ; Godbout N ; Sampson DD ; Boudoux C ; McLaughlin RA
  • Biomed Opt Express 2015[May]; 6 (5 ): 1767-81 PMID26137379 show ga
  • Molecular imaging using optical techniques provides insight into disease at the cellular level. In this paper, we report on a novel dual-modality probe capable of performing molecular imaging by combining simultaneous three-dimensional optical coherence tomography (OCT) and two-dimensional fluorescence imaging in a hypodermic needle. The probe, referred to as a molecular imaging (MI) needle, may be inserted tens of millimeters into tissue. The MI needle utilizes double-clad fiber to carry both imaging modalities, and is interfaced to a 1310-nm OCT system and a fluorescence imaging subsystem using an asymmetrical double-clad fiber coupler customized to achieve high fluorescence collection efficiency. We present, to the best of our knowledge, the first dual-modality OCT and fluorescence needle probe with sufficient sensitivity to image fluorescently labeled antibodies. Such probes enable high-resolution molecular imaging deep within tissue.
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