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10.1186/s12967-015-0536-7 http://scihub22266oqcxt.onion/10.1186/s12967-015-0536-7 C4467615!4467615 !26040555     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26040555 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       J+Transl+Med 2015 ; 13 (ä): 178 Nephropedia Template TP {{tp|p=26040555 |t=2015. Anti-dsDNA antibodies induce inflammation via endoplasmic reticulum stress in human mesangial cells |pdf=|usr=}}{{26040555 }} gab.com Text Anti-dsDNA antibodies induce inflammation via endoplasmic reticulum stress in human mesangial cells JO: J Transl Med http://www.kidney.de/pget.php?&tw=1&pmid=26040555 #FOAvip BACKGROUND: Anti-dsDNA antibodies play an important role in the pathogenesis of lupus nephritis (LN). Endoplasmic reticulum (ER) stress is a physical reaction under stressful condition and can cause inflammation when stimulation is sustained. This study investigated the roles of ER stress in anti-dsDNA antibody-induced inflammation response in human mesangial cells (HMCs). METHOD: Anti-dsDNA antibodies isolated from LN patients were used to stimulate HMCs. The expression of GRP78, PERK, p-PERK, p-eIF2?, ATF4, p-IRE1?, ATF6 and CHOP in HMCs was measured by western blot. NF-?B activation was detected by examining nuclear translocation of NF-?B p65. The expression and production of IL-1?, TNF-? and MCP-1 were examined by qPCR and ELISA. RESULTS: Flow cytometry and cellular ELISA showed that anti-dsDNA antibodies can bind to HMCs. The binding was not inhibited by blockage of Fc receptor. Anti-dsDNA antibody stimulation significantly enhanced the expression of GRP78, p-PERK, p-eIF2? and ATF4 in HMCs. However, no significant increase in the expression of p-IRE1? and ATF6 was found. In addition, anti-dsDNA antibodies also significantly increased the activation of NF-?B and upregulated the expression of IL-1?, TNF-? and MCP-1, which were suppressed by pretreatment of HMCs with chemical ER stress inhibitor 4-PBA. Transfection of specific ATF4 siRNA also significantly reduced the activation of NF-?B and expression of proinflammatory cytokines. CONCLUSION: Anti-dsDNA antibodies induce NF-?B activation and inflammation in HMCs via PERK-eIF2?-ATF4 ER stress pathway. Twit Text FOAVip Anti-dsDNA antibodies induce inflammation via endoplasmic reticulum stress in human mesangial cells ????J Transl Med http://www.kidney.de/pget.php?&tw=1&pmid=26040555 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26040555 #FOAvip English Wikipedia <ref>{{Cite pmid|26040555 }}</ref> Anti-dsDNA antibodies induce inflammation via endoplasmic reticulum stress in human mesangial cells #MMPMID26040555 Zhang H ; Zhao C ; Wang S ; Huang Y ; Wang H ; Zhao J ; Yang N J Transl Med 2015[Jun]; 13 (ä): 178 PMID26040555 show ga BACKGROUND: Anti-dsDNA antibodies play an important role in the pathogenesis of lupus nephritis (LN). Endoplasmic reticulum (ER) stress is a physical reaction under stressful condition and can cause inflammation when stimulation is sustained. This study investigated the roles of ER stress in anti-dsDNA antibody-induced inflammation response in human mesangial cells (HMCs). METHOD: Anti-dsDNA antibodies isolated from LN patients were used to stimulate HMCs. The expression of GRP78, PERK, p-PERK, p-eIF2?, ATF4, p-IRE1?, ATF6 and CHOP in HMCs was measured by western blot. NF-?B activation was detected by examining nuclear translocation of NF-?B p65. The expression and production of IL-1?, TNF-? and MCP-1 were examined by qPCR and ELISA. RESULTS: Flow cytometry and cellular ELISA showed that anti-dsDNA antibodies can bind to HMCs. The binding was not inhibited by blockage of Fc receptor. Anti-dsDNA antibody stimulation significantly enhanced the expression of GRP78, p-PERK, p-eIF2? and ATF4 in HMCs. However, no significant increase in the expression of p-IRE1? and ATF6 was found. In addition, anti-dsDNA antibodies also significantly increased the activation of NF-?B and upregulated the expression of IL-1?, TNF-? and MCP-1, which were suppressed by pretreatment of HMCs with chemical ER stress inhibitor 4-PBA. Transfection of specific ATF4 siRNA also significantly reduced the activation of NF-?B and expression of proinflammatory cytokines. CONCLUSION: Anti-dsDNA antibodies induce NF-?B activation and inflammation in HMCs via PERK-eIF2?-ATF4 ER stress pathway. |Activating Transcription Factor 6/metabolism [MESH] |Adolescent [MESH] |Adult [MESH] |Antibodies, Antinuclear/pharmacology [MESH] |Demography [MESH] |Endoplasmic Reticulum Chaperone BiP [MESH] |Endoplasmic Reticulum Stress/*drug effects [MESH] |Endoribonucleases/metabolism [MESH] |Female [MESH] |Humans [MESH] |Inflammation/*pathology [MESH] |Male [MESH] |Mesangial Cells/drug effects/*pathology [MESH] |Middle Aged [MESH] |NF-kappa B/metabolism [MESH] |Protein Binding/drug effects [MESH] |Protein Serine-Threonine Kinases/metabolism [MESH] |Up-Regulation/drug effects [MESH] |Young Adult [MESH]Anti-dsDNA antibodies induce inflammation via endoplasmic reticulum st(epmc).pdf DeepDyve Pubget Overpricing