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10.1016/j.pep.2014.08.010 http://scihub22266oqcxt.onion/10.1016/j.pep.2014.08.010 C4466898!4466898!25149396     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Protein+Expr+Purif 2014 ; 103 (ä): 75-83 Nephropedia Template TP {{tp|p=25149396|t=2014. Production and characterization of thirteen human Type-I interferon-? subtypes |pdf=|usr=}}{{25149396}} gab.com Text Production and characterization of thirteen human Type-I interferon- subtypes JO: Protein Expr Purif http://www.kidney.de/pget.php?&tw=1&pmid=25149396 #FOAvip Thirteen human interferon-? (IFN?) subtypes were expressed in E. coli and purified using an N-terminal affinity tag from the prodomain of subtilisn. IFN? subtypes were expressed in soluble form and purified from cell lysates or refolded and purified from inclusion bodies. Proteins produced by either protocol exhibited biological activities equal to or greater than commercially prepared IFN? preparations. The IFN?s were used to produce an anti-IFN?16 antibody (MAb-1B12) that specifically neutralized the biological activity of IFN?16, but not the 12 other IFN?s. Using MAb-1B12, and a previously generated IFNAR1/IFNAR2-FChk heterodimer, an assay was developed to determine total type I IFN biological activity and IFN?16-derived biological activity in an unknown sample. Twit Text FOAVip Production and characterization of thirteen human Type-I interferon- subtypes ????Protein Expr Purif http://www.kidney.de/pget.php?&tw=1&pmid=25149396 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25149396 #FOAvip English Wikipedia <ref>{{Cite pmid|25149396}}</ref> Production and characterization of thirteen human Type-I interferon-? subtypes #MMPMID25149396 Kuruganti S; Accavitti-Loper MA; Walter MR Protein Expr Purif 2014[Nov]; 103 (ä): 75-83 PMID25149396show ga Thirteen human interferon-? (IFN?) subtypes were expressed in E. coli and purified using an N-terminal affinity tag from the prodomain of subtilisn. IFN? subtypes were expressed in soluble form and purified from cell lysates or refolded and purified from inclusion bodies. Proteins produced by either protocol exhibited biological activities equal to or greater than commercially prepared IFN? preparations. The IFN?s were used to produce an anti-IFN?16 antibody (MAb-1B12) that specifically neutralized the biological activity of IFN?16, but not the 12 other IFN?s. Using MAb-1B12, and a previously generated IFNAR1/IFNAR2-FChk heterodimer, an assay was developed to determine total type I IFN biological activity and IFN?16-derived biological activity in an unknown sample. äProduction and characterization of thirteen human Type-I interferon-? (epmc).pdf DeepDyve Pubget Overpricing