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10.1038/srep11339 http://scihub22266oqcxt.onion/10.1038/srep11339 C4466892!4466892 !26074253     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26074253 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Sci+Rep 2015 ; 5 (ä): 11339 Nephropedia Template TP {{tp|p=26074253 |t=2015. Rapid, automated, parallel quantitative immunoassays using highly integrated microfluidics and AlphaLISA |pdf=|usr=}}{{26074253 }} gab.com Text Rapid, automated, parallel quantitative immunoassays using highly integrated microfluidics and AlphaLISA JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26074253 #FOAvip Immunoassays represent one of the most popular analytical methods for detection and quantification of biomolecules. However, conventional immunoassays such as ELISA and flow cytometry, even though providing high sensitivity and specificity and multiplexing capability, can be labor-intensive and prone to human error, making them unsuitable for standardized clinical diagnoses. Using a commercialized no-wash, homogeneous immunoassay technology ('AlphaLISA') in conjunction with integrated microfluidics, herein we developed a microfluidic immunoassay chip capable of rapid, automated, parallel immunoassays of microliter quantities of samples. Operation of the microfluidic immunoassay chip entailed rapid mixing and conjugation of AlphaLISA components with target analytes before quantitative imaging for analyte detections in up to eight samples simultaneously. Aspects such as fluid handling and operation, surface passivation, imaging uniformity, and detection sensitivity of the microfluidic immunoassay chip using AlphaLISA were investigated. The microfluidic immunoassay chip could detect one target analyte simultaneously for up to eight samples in 45 min with a limit of detection down to 10 pg mL(-1). The microfluidic immunoassay chip was further utilized for functional immunophenotyping to examine cytokine secretion from human immune cells stimulated ex vivo. Together, the microfluidic immunoassay chip provides a promising high-throughput, high-content platform for rapid, automated, parallel quantitative immunosensing applications. Twit Text FOAVip Rapid, automated, parallel quantitative immunoassays using highly integrated microfluidics and AlphaLISA ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26074253 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26074253 #FOAvip English Wikipedia <ref>{{Cite pmid|26074253 }}</ref> Rapid, automated, parallel quantitative immunoassays using highly integrated microfluidics and AlphaLISA #MMPMID26074253 Yu ZT ; Guan H ; Cheung MK ; McHugh WM ; Cornell TT ; Shanley TP ; Kurabayashi K ; Fu J Sci Rep 2015[Jun]; 5 (ä): 11339 PMID26074253 show ga Immunoassays represent one of the most popular analytical methods for detection and quantification of biomolecules. However, conventional immunoassays such as ELISA and flow cytometry, even though providing high sensitivity and specificity and multiplexing capability, can be labor-intensive and prone to human error, making them unsuitable for standardized clinical diagnoses. Using a commercialized no-wash, homogeneous immunoassay technology ('AlphaLISA') in conjunction with integrated microfluidics, herein we developed a microfluidic immunoassay chip capable of rapid, automated, parallel immunoassays of microliter quantities of samples. Operation of the microfluidic immunoassay chip entailed rapid mixing and conjugation of AlphaLISA components with target analytes before quantitative imaging for analyte detections in up to eight samples simultaneously. Aspects such as fluid handling and operation, surface passivation, imaging uniformity, and detection sensitivity of the microfluidic immunoassay chip using AlphaLISA were investigated. The microfluidic immunoassay chip could detect one target analyte simultaneously for up to eight samples in 45 min with a limit of detection down to 10 pg mL(-1). The microfluidic immunoassay chip was further utilized for functional immunophenotyping to examine cytokine secretion from human immune cells stimulated ex vivo. Together, the microfluidic immunoassay chip provides a promising high-throughput, high-content platform for rapid, automated, parallel quantitative immunosensing applications. |*Lab-On-A-Chip Devices [MESH] |*Microfluidic Analytical Techniques [MESH] |Antibodies, Monoclonal/chemistry [MESH] |Automation, Laboratory [MESH] |Cell Line, Tumor [MESH] |Equipment Design [MESH] |Humans [MESH] |Immunoassay/instrumentation/*methods [MESH] |Leukocytes, Mononuclear/cytology/drug effects/immunology [MESH] |Limit of Detection [MESH] |Lipopolysaccharides/pharmacology [MESH] |Primary Cell Culture [MESH] |Reproducibility of Results [MESH]Rapid, automated, parallel quantitative immunoassays using highly inte(epmc).pdf DeepDyve Pubget Overpricing