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10.1111/1462-2920.12095 http://scihub22266oqcxt.onion/10.1111/1462-2920.12095 C4466117!4466117 !23425591     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=23425591 &cmd=llinks): Failed to open stream: HTTP request failed! 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A spider web strategy of type IV pili-mediated migration to build a fibre-like Psl polysaccharide matrix in Pseudomonas aeruginosa biofilms |pdf=|usr=}}{{23425591 }} gab.com Text A spider web strategy of type IV pili-mediated migration to build a fibre-like Psl polysaccharide matrix in Pseudomonas aeruginosa biofilms JO: Environ Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=23425591 #FOAvip Bacterial motilities participate in biofilm development. However, it is unknown how/if bacterial motility affects formation of the biofilm matrix. Psl polysaccharide is a key biofilm matrix component of Pseudomonas aeruginosa. Here we report that type IV pili (T4P)-mediated bacterial migration leads to the formation of a fibre-like Psl matrix. Deletion of T4P in wild type and flagella-deficient strains results in loss of the Psl-fibres and reduction of biofilm biomass in flow cell biofilms as well as pellicles at air-liquid interface. Bacteria lacking T4P-driven twitching motility including those that still express surface T4P are unable to form the Psl-fibres. Formation of a Psl-fibre matrix is critical for efficient biofilm formation, yet does not require flagella and polysaccharide Pel or alginate. The Psl-fibres are likely formed by Psl released from bacteria during T4P-mediated migration, a strategy similar to spider web formation. Starvation can couple Psl release and T4P-driven twitching motility. Furthermore, a radial-pattern Psl-fibre matrix is present in the middle of biofilms, a nutrient-deprived region. These imply a plausible model for how bacteria respond to nutrient-limited local environment to build a polysaccharide-fibre matrix by T4P-dependent bacterial migration strategy. This strategy may have general significance for bacterial survival in natural and clinical settings. Twit Text FOAVip A spider web strategy of type IV pili-mediated migration to build a fibre-like Psl polysaccharide matrix in Pseudomonas aeruginosa biofilms ????Environ Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=23425591 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=23425591 #FOAvip English Wikipedia <ref>{{Cite pmid|23425591 }}</ref> A spider web strategy of type IV pili-mediated migration to build a fibre-like Psl polysaccharide matrix in Pseudomonas aeruginosa biofilms #MMPMID23425591 Wang S ; Parsek MR ; Wozniak DJ ; Ma LZ Environ Microbiol 2013[Aug]; 15 (8 ): 2238-53 PMID23425591 show ga Bacterial motilities participate in biofilm development. However, it is unknown how/if bacterial motility affects formation of the biofilm matrix. Psl polysaccharide is a key biofilm matrix component of Pseudomonas aeruginosa. Here we report that type IV pili (T4P)-mediated bacterial migration leads to the formation of a fibre-like Psl matrix. Deletion of T4P in wild type and flagella-deficient strains results in loss of the Psl-fibres and reduction of biofilm biomass in flow cell biofilms as well as pellicles at air-liquid interface. Bacteria lacking T4P-driven twitching motility including those that still express surface T4P are unable to form the Psl-fibres. Formation of a Psl-fibre matrix is critical for efficient biofilm formation, yet does not require flagella and polysaccharide Pel or alginate. The Psl-fibres are likely formed by Psl released from bacteria during T4P-mediated migration, a strategy similar to spider web formation. Starvation can couple Psl release and T4P-driven twitching motility. Furthermore, a radial-pattern Psl-fibre matrix is present in the middle of biofilms, a nutrient-deprived region. These imply a plausible model for how bacteria respond to nutrient-limited local environment to build a polysaccharide-fibre matrix by T4P-dependent bacterial migration strategy. This strategy may have general significance for bacterial survival in natural and clinical settings. |*Environmental Microbiology [MESH] |Alginates/metabolism [MESH] |Animals [MESH] |Biofilms/*growth & development [MESH] |Fimbriae, Bacterial/genetics/metabolism [MESH] |Flagella/genetics/metabolism [MESH] |Glucuronic Acid/metabolism [MESH] |Hexuronic Acids/metabolism [MESH] |Polysaccharides, Bacterial/genetics/*metabolism [MESH] |Pseudomonas aeruginosa/genetics/metabolism/*physiology [MESH]A spider web strategy of type IV pili-mediated migration to build a fi(epmc).pdf DeepDyve Pubget Overpricing