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10.1016/j.bbagen.2013.07.026

http://scihub22266oqcxt.onion/10.1016/j.bbagen.2013.07.026
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C4465751!4465751!23988402
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suck abstract from ncbi

pmid23988402      Biochim+Biophys+Acta 2014 ; 1840 (2): 892-900
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  • Detection of S-Nitrosothiols #MMPMID23988402
  • Diers AR; Keszler A; Hogg N
  • Biochim Biophys Acta 2014[Feb]; 1840 (2): 892-900 PMID23988402show ga
  • BACKGROUND: S-Nitrosothiols have been recognized as biologically-relevant products of nitric oxide that are involved in many of the diverse activities of this free radical. SCOPE OF REVIEW: This review serves to discuss current methods for the detection and analysis of protein S-nitrosothiols. The major methods of S-nitrosothiol detection include chemiluminescence-based methods and switch-based methods, each of which comes in various flavors with advantages and caveats. MAJOR CONCLUSIONS: The detection of S-nitrosothiols is challenging and prone to many artifacts. Accurate measurements require an understanding of the underlying chemistry of the methods involved and the use of appropriate controls. GENERAL SIGNIFICANCE: Nothing is more important to a field of research than robust methodology that is generally trusted. The field of S-Nitrosation has developed such methods but, as S-nitrosothiols are easy to introduce as artifacts, it is vital that current users learn from the lessons of the past.
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