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10.1007/978-1-61779-851-1_27 http://scihub22266oqcxt.onion/10.1007/978-1-61779-851-1_27 C4465354!4465354!22639272     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Methods+Mol+Biol 2012 ; 886 (ä): 305-9 Nephropedia Template TP {{tp|p=22639272|t=2012. Morpholino-Mediated Gene Knockdown in Mammalian Organ Culture |pdf=|usr=}}{{22639272}} gab.com Text Morpholino-Mediated Gene Knockdown in Mammalian Organ Culture JO: Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=22639272 #FOAvip We examined the role of semaphorin3a in ureteric bud (UB) branching morphogenesis using mouse metanephric organ culture [Tufro et al. (Mech Dev 125:558?568, 2008)]. In vitro UB injection of Sema3a antisense morpholino resulted in increased branching morphogenesis. Cellular and tissue uptake of oligonucleotides was facilitated by a peptide-mediated method. Our findings were validated by in vitro translation and in Sema3a null mice. This chapter describes a method to perfuse the UB lumen with fluorescein-labeled oligonucleotides bound to a peptide carrier. Twit Text FOAVip Morpholino-Mediated Gene Knockdown in Mammalian Organ Culture ????Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=22639272 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=22639272 #FOAvip English Wikipedia <ref>{{Cite pmid|22639272}}</ref> Morpholino-Mediated Gene Knockdown in Mammalian Organ Culture #MMPMID22639272 Tufro A Methods Mol Biol 2012[]; 886 (ä): 305-9 PMID22639272show ga We examined the role of semaphorin3a in ureteric bud (UB) branching morphogenesis using mouse metanephric organ culture [Tufro et al. (Mech Dev 125:558?568, 2008)]. In vitro UB injection of Sema3a antisense morpholino resulted in increased branching morphogenesis. Cellular and tissue uptake of oligonucleotides was facilitated by a peptide-mediated method. Our findings were validated by in vitro translation and in Sema3a null mice. This chapter describes a method to perfuse the UB lumen with fluorescein-labeled oligonucleotides bound to a peptide carrier. äMorpholino-Mediated Gene Knockdown in Mammalian Organ Culture (epmc).pdf DeepDyve Pubget Overpricing