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10.1038/srep11019 http://scihub22266oqcxt.onion/10.1038/srep11019 C4464258!4464258 !26066093     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26066093 &cmd=llinks): Failed to open stream: HTTP request failed! 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Generation of pure lymphatic endothelial cells from human pluripotent stem cells and their therapeutic effects on wound repair |pdf=|usr=}}{{26066093 }} gab.com Text Generation of pure lymphatic endothelial cells from human pluripotent stem cells and their therapeutic effects on wound repair JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26066093 #FOAvip Human pluripotent stem cells (hPSCs) have emerged as an important source for cell therapy. However, to date, no studies demonstrated generation of purified hPSC-derived lymphatic endothelial cells (LECs) and tested their therapeutic potential in disease models. Here we sought to differentiate hPSCs into the LEC lineage, purify them with LEC markers, and evaluate their therapeutic effects. We found that an OP9-assisted culture system reinforced by addition of VEGF-A, VEGF-C, and EGF most efficiently generated LECs, which were then isolated via FACS-sorting with LYVE-1 and PODOPLANIN. These hPSC-derived LYVE-1(+)PODOPLANIN(+)cells showed a pure committed LEC phenotype, formed new lymphatic vessels, and expressed lymphangiogenic factors at high levels. These hPSC-derived LECs enhanced wound healing through lymphangiogenesis and lymphvasculogenesis. Here we report, for the first time, that LECs can be selectively isolated from differentiating hPSCs, and that these cells are potent for lymphatic vessel formation in vivo and wound healing. This system and the purified hPSC-derived LECs can serve as a new platform for studying LEC development as well as for cell therapy. Twit Text FOAVip Generation of pure lymphatic endothelial cells from human pluripotent stem cells and their therapeutic effects on wound repair ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26066093 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26066093 #FOAvip English Wikipedia <ref>{{Cite pmid|26066093 }}</ref> Generation of pure lymphatic endothelial cells from human pluripotent stem cells and their therapeutic effects on wound repair #MMPMID26066093 Lee SJ ; Park C ; Lee JY ; Kim S ; Kwon PJ ; Kim W ; Jeon YH ; Lee E ; Yoon YS Sci Rep 2015[Jun]; 5 (ä): 11019 PMID26066093 show ga Human pluripotent stem cells (hPSCs) have emerged as an important source for cell therapy. However, to date, no studies demonstrated generation of purified hPSC-derived lymphatic endothelial cells (LECs) and tested their therapeutic potential in disease models. Here we sought to differentiate hPSCs into the LEC lineage, purify them with LEC markers, and evaluate their therapeutic effects. We found that an OP9-assisted culture system reinforced by addition of VEGF-A, VEGF-C, and EGF most efficiently generated LECs, which were then isolated via FACS-sorting with LYVE-1 and PODOPLANIN. These hPSC-derived LYVE-1(+)PODOPLANIN(+)cells showed a pure committed LEC phenotype, formed new lymphatic vessels, and expressed lymphangiogenic factors at high levels. These hPSC-derived LECs enhanced wound healing through lymphangiogenesis and lymphvasculogenesis. Here we report, for the first time, that LECs can be selectively isolated from differentiating hPSCs, and that these cells are potent for lymphatic vessel formation in vivo and wound healing. This system and the purified hPSC-derived LECs can serve as a new platform for studying LEC development as well as for cell therapy. |*Cell Differentiation [MESH] |*Cell- and Tissue-Based Therapy [MESH] |*Lymphangiogenesis [MESH] |*Wound Healing [MESH] |Animals [MESH] |Endothelial Cells/cytology/*metabolism/transplantation [MESH] |Epidermal Growth Factor/pharmacology [MESH] |Heterografts [MESH] |Humans [MESH] |Mice [MESH] |Mice, Nude [MESH] |Pluripotent Stem Cells [MESH] |Vascular Endothelial Growth Factor A/pharmacology [MESH]Generation of pure lymphatic endothelial cells from human pluripotent (epmc).pdf DeepDyve Pubget Overpricing