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pmid26085952      Acta+Naturae 2015 ; 7 (2): 108-14
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  • The Use of Atomic Force Microscopy for 3D Analysis of Nucleic Acid Hybridization on Microarrays #MMPMID26085952
  • Dubrovin EV; Presnova GV; Rubtsova MY; Egorov AM; Grigorenko VG; Yaminsky IV
  • Acta Naturae 2015[Apr]; 7 (2): 108-14 PMID26085952show ga
  • Oligonucleotide microarrays are considered today to be one of the most efficient methods of gene diagnostics. The capability of atomic force microscopy (AFM) to characterize the three-dimensional morphology of single molecules on a surface allows one to use it as an effective tool for the 3D analysis of a microarray for the detection of nucleic acids. The high resolution of AFM offers ways to decrease the detection threshold of target DNA and increase the signal-to-noise ratio. In this work, we suggest an approach to the evaluation of the results of hybridization of gold nanoparticle-labeled nucleic acids on silicon microarrays based on an AFM analysis of the surface both in air and in liquid which takes into account of their three-dimensional structure. We suggest a quantitative measure of the hybridization results which is based on the fraction of the surface area occupied by the nanoparticles.
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