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10.5935/1678-9741.20150016 http://scihub22266oqcxt.onion/10.5935/1678-9741.20150016 C4462960!4462960 !26107446     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\26107446 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Rev+Bras+Cir+Cardiovasc 2015 ; 30 (2 ): 159-63 Nephropedia Template TP {{tp|p=26107446 |t=2015. Study of novel coating strategy for coronary stents: simutaneous coating of VEGF and anti- CD34 antibody |pdf=|usr=}}{{26107446 }} gab.com Text Study of novel coating strategy for coronary stents: simutaneous coating of VEGF and anti- CD34 antibody JO: Rev Bras Cir Cardiovasc http://www.kidney.de/pget.php?&tw=1&pmid=26107446 #FOAvip INTRODUCTION: Intravascular coronary stenting has been used in the treatment of coronary artery disease (CAD), with a major limitation of in-stent restenosis (ISR). The 316 stainless steel has been widely used for coronary stents. In this study, we developed a novel coating method to reduce ISR by simultaneously coating vascular endothelial growth factor (VEGF) and anti-CD34 antibody on 316L stainless steel. METHODS: Round 316L stainless steel sheets in the D-H group were polymerized with compounds generated from condensation reaction of dopamine and heparin using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC) and N-hydroxysuccinimide (NHS). Sixteen sheets from the D-H group were further immersed into 1ug/ml VEGF165 and 3mg/ml heparin sodium one after another for 10 times, and named as the D-(H-V)10 group. Eight sheets from the D-(H-V)10 group were coated with anti-CD34 antibody and termed as the D-(H-V)10-A group. Immunofluorescence assay and ELISA were used to evaluate whether the 316L stainless steel disks were successfully coated with VEGF and anti-CD34 antibody. RESULTS: The results of immunofluorescence assay and ELISA showed that VEGF could be detected in the D-(H-V)10 and D-(H-V)10-A group, suggesting the steel sheets were successfully covered with VEGF. Anti-CD34 antibody could only be observed in the D-(H-V)10-A group, which was the only group coated with CD34 antibody. Both results suggested that the 316L stainless steel sheets were successfully coated with VEGF and anti-CD34 antibody. CONCLUSION: Our study developed a method to simultaneously coat VEGF and anti-CD34 antibody to stainless metal steel. This research serves as a fundamental role for a novel coating strategy. Twit Text FOAVip Study of novel coating strategy for coronary stents: simutaneous coating of VEGF and anti- CD34 antibody ????Rev Bras Cir Cardiovasc http://www.kidney.de/pget.php?&tw=1&pmid=26107446 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26107446 #FOAvip English Wikipedia <ref>{{Cite pmid|26107446 }}</ref> Study of novel coating strategy for coronary stents: simutaneous coating of VEGF and anti- CD34 antibody #MMPMID26107446 Song CL ; Li Q ; Yu YP ; Wang G ; Wang JP ; Lu Y ; Zhang JC ; Diao HY ; Liu JG ; Liu YH ; Liu J ; Li Y ; Cai D ; Liu B Rev Bras Cir Cardiovasc 2015[Mar]; 30 (2 ): 159-63 PMID26107446 show ga INTRODUCTION: Intravascular coronary stenting has been used in the treatment of coronary artery disease (CAD), with a major limitation of in-stent restenosis (ISR). The 316 stainless steel has been widely used for coronary stents. In this study, we developed a novel coating method to reduce ISR by simultaneously coating vascular endothelial growth factor (VEGF) and anti-CD34 antibody on 316L stainless steel. METHODS: Round 316L stainless steel sheets in the D-H group were polymerized with compounds generated from condensation reaction of dopamine and heparin using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC) and N-hydroxysuccinimide (NHS). Sixteen sheets from the D-H group were further immersed into 1ug/ml VEGF165 and 3mg/ml heparin sodium one after another for 10 times, and named as the D-(H-V)10 group. Eight sheets from the D-(H-V)10 group were coated with anti-CD34 antibody and termed as the D-(H-V)10-A group. Immunofluorescence assay and ELISA were used to evaluate whether the 316L stainless steel disks were successfully coated with VEGF and anti-CD34 antibody. RESULTS: The results of immunofluorescence assay and ELISA showed that VEGF could be detected in the D-(H-V)10 and D-(H-V)10-A group, suggesting the steel sheets were successfully covered with VEGF. Anti-CD34 antibody could only be observed in the D-(H-V)10-A group, which was the only group coated with CD34 antibody. Both results suggested that the 316L stainless steel sheets were successfully coated with VEGF and anti-CD34 antibody. CONCLUSION: Our study developed a method to simultaneously coat VEGF and anti-CD34 antibody to stainless metal steel. This research serves as a fundamental role for a novel coating strategy. |*Drug-Eluting Stents [MESH] |Antigens, CD34/*chemistry/*immunology [MESH] |Coated Materials, Biocompatible/*chemistry [MESH] |Coronary Restenosis/prevention & control [MESH] |Enzyme-Linked Immunosorbent Assay [MESH] |Ethyldimethylaminopropyl Carbodiimide/chemistry [MESH] |Fluorescent Antibody Technique [MESH] |Humans [MESH] |Materials Testing [MESH] |Reproducibility of Results [MESH] |Serum Albumin, Bovine [MESH] |Stainless Steel/*chemistry [MESH] |Time Factors [MESH]Study of novel coating strategy for coronary stents: simutaneous coati(epmc).pdf DeepDyve Pubget Overpricing