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10.1016/j.canlet.2014.09.031 http://scihub22266oqcxt.onion/10.1016/j.canlet.2014.09.031 C4462172!4462172 !25304374     free     free     free Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 249.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 249.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 249.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25304374 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Cancer+Lett 2014 ; 355 (2 ): 273-80 Nephropedia Template TP {{tp|p=25304374 |t=2014. Knockdown or inhibition of aldo-keto reductase 1B10 inhibits pancreatic carcinoma growth via modulating Kras-E-cadherin pathway |pdf=|usr=}}{{25304374 }} gab.com Text Knockdown or inhibition of aldo-keto reductase 1B10 inhibits pancreatic carcinoma growth via modulating Kras-E-cadherin pathway JO: Cancer Lett http://www.kidney.de/pget.php?&tw=1&pmid=25304374 #FOAvip Aldo-keto reductase 1B10 (AKR1B10) has relatively specific lipid substrates including carbonyls, retinal and farnesal/geranylgeranial. Metabolizing these lipid substrates appears crucial to carcinogenesis, particularly for farnesal/geranylgeranial that involves protein prenylation. Mutant Kras is a most common active oncogene in pancreatic cancer, and its activation requires protein prenylation. To directly determine the role of AKR1B10 in pancreatic carcinogenesis, we knocked down AKR1B10 in CD18 human pancreatic carcinoma cells using shRNA approach. Silencing AKR1B10 resulted in a significant inhibition of anchor-dependent growth (knockdown cells vs. vector-control cells: 67?±?9.5 colonies/HPF vs. 170?±?3.7 colonies/HPF, p?<?0.01), invasion index (0.27 vs. 1.00, p?<?0.05), and cell migration (at 16 hours 9.2?±?1.2% vs. 14.0?±?1.8%, at 24 hours 21.0?±?1.1% vs. 30.5?±?3.5%, and at 48 hours 51.9?±?5.7% vs. 88.9?±?3.0%, p?<?0.01). Inhibition of AKR1B10 by oleanolic acid (OA) showed a dose-dependent inhibition of cell growth with IC50 at 30?µM. Kras pull-down and Western blot analysis revealed a significant down-regulation of active form Kras and phosphorylated C-Raf, and Erk, as well as an up-regulation of E-cadherin. A significant reduction of in vivo tumor growth was observed in nude mice implanted with the CD18 pancreatic carcinoma cells with AKR1B10 knockdown (tumor weight: 0.25?±?0.06?g vs. 0.52?±?0.07?g, p?=?0.01), and with OA treatment (tumor weight: 0.35?±?0.05?g vs. 0.52?±?0.07?g, p?=?0.05). Our findings indicate AKR1B10 is a unique enzyme involved in pancreatic carcinogenesis via modulation of the Kras-E-cadherin pathway. Twit Text FOAVip Knockdown or inhibition of aldo-keto reductase 1B10 inhibits pancreatic carcinoma growth via modulating Kras-E-cadherin pathway ????Cancer Lett http://www.kidney.de/pget.php?&tw=1&pmid=25304374 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25304374 #FOAvip English Wikipedia <ref>{{Cite pmid|25304374 }}</ref> Knockdown or inhibition of aldo-keto reductase 1B10 inhibits pancreatic carcinoma growth via modulating Kras-E-cadherin pathway #MMPMID25304374 Zhang W ; Li H ; Yang Y ; Liao J ; Yang GY Cancer Lett 2014[Dec]; 355 (2 ): 273-80 PMID25304374 show ga Aldo-keto reductase 1B10 (AKR1B10) has relatively specific lipid substrates including carbonyls, retinal and farnesal/geranylgeranial. Metabolizing these lipid substrates appears crucial to carcinogenesis, particularly for farnesal/geranylgeranial that involves protein prenylation. Mutant Kras is a most common active oncogene in pancreatic cancer, and its activation requires protein prenylation. To directly determine the role of AKR1B10 in pancreatic carcinogenesis, we knocked down AKR1B10 in CD18 human pancreatic carcinoma cells using shRNA approach. Silencing AKR1B10 resulted in a significant inhibition of anchor-dependent growth (knockdown cells vs. vector-control cells: 67?±?9.5 colonies/HPF vs. 170?±?3.7 colonies/HPF, p? |Aldehyde Reductase/*antagonists & inhibitors/*genetics [MESH] |Aldo-Keto Reductases [MESH] |Animals [MESH] |CD18 Antigens/genetics [MESH] |Cadherins/genetics/*metabolism [MESH] |Cell Line, Tumor [MESH] |Down-Regulation [MESH] |Enzyme Inhibitors/pharmacology [MESH] |Gene Expression Regulation, Neoplastic [MESH] |Gene Knockdown Techniques [MESH] |Genes, ras [MESH] |Humans [MESH] |Mice [MESH] |Mice, Inbred BALB C [MESH] |Mice, Nude [MESH] |Oleanolic Acid/*pharmacology [MESH] |Pancreatic Neoplasms/genetics/*metabolism/pathology/*therapy [MESH] |Proto-Oncogene Proteins p21(ras) [MESH] |Proto-Oncogene Proteins/genetics/*metabolism [MESH] |RNA, Small Interfering/administration & dosage/genetics [MESH] |Signal Transduction [MESH] |Transfection [MESH] |Up-Regulation [MESH] |Xenograft Model Antitumor Assays [MESH]Knockdown or inhibition of aldo-keto reductase 1B10 inhibits pancreati(epmc).pdf DeepDyve Pubget Overpricing