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10.1186/s13073-015-0178-7 http://scihub22266oqcxt.onion/10.1186/s13073-015-0178-7 C4460969!4460969!26060510     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Genome+Med 2015 ; 7 (1): ä Nephropedia Template TP {{tp|p=26060510|t=2015. Precision cancer mouse models through genome editing with CRISPR-Cas9 |pdf=|usr=}}{{26060510}} gab.com Text Precision cancer mouse models through genome editing with CRISPR-Cas9 JO: Genome Med http://www.kidney.de/pget.php?&tw=1&pmid=26060510 #FOAvip The cancer genome is highly complex, with hundreds of point mutations, translocations, and chromosome gains and losses per tumor. To understand the effects of these alterations, precise models are needed. Traditional approaches to the construction of mouse models are time-consuming and laborious, requiring manipulation of embryonic stem cells and multiple steps. The recent development of the clustered regularly interspersed short palindromic repeats (CRISPR)-Cas9 system, a powerful genome-editing tool for efficient and precise genome engineering in cultured mammalian cells and animals, is transforming mouse-model generation. Here, we review how CRISPR-Cas9 has been used to create germline and somatic mouse models with point mutations, deletions and complex chromosomal rearrangements. We highlight the progress and challenges of such approaches, and how these models can be used to understand the evolution and progression of individual tumors and identify new strategies for cancer treatment. The generation of precision cancer mouse models through genome editing will provide a rapid avenue for functional cancer genomics and pave the way for precision cancer medicine. Twit Text FOAVip Precision cancer mouse models through genome editing with CRISPR-Cas9 ????Genome Med http://www.kidney.de/pget.php?&tw=1&pmid=26060510 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26060510 #FOAvip English Wikipedia <ref>{{Cite pmid|26060510}}</ref> Precision cancer mouse models through genome editing with CRISPR-Cas9 #MMPMID26060510 Mou H; Kennedy Z; Anderson DG; Yin H; Xue W Genome Med 2015[]; 7 (1): ä PMID26060510show ga The cancer genome is highly complex, with hundreds of point mutations, translocations, and chromosome gains and losses per tumor. To understand the effects of these alterations, precise models are needed. Traditional approaches to the construction of mouse models are time-consuming and laborious, requiring manipulation of embryonic stem cells and multiple steps. The recent development of the clustered regularly interspersed short palindromic repeats (CRISPR)-Cas9 system, a powerful genome-editing tool for efficient and precise genome engineering in cultured mammalian cells and animals, is transforming mouse-model generation. Here, we review how CRISPR-Cas9 has been used to create germline and somatic mouse models with point mutations, deletions and complex chromosomal rearrangements. We highlight the progress and challenges of such approaches, and how these models can be used to understand the evolution and progression of individual tumors and identify new strategies for cancer treatment. The generation of precision cancer mouse models through genome editing will provide a rapid avenue for functional cancer genomics and pave the way for precision cancer medicine. äPrecision cancer mouse models through genome editing with CRISPR-Cas9 (epmc).pdf DeepDyve Pubget Overpricing