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10.1083/jcb.201409074

http://scihub22266oqcxt.onion/10.1083/jcb.201409074
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C4460151!4460151!26056137
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suck abstract from ncbi

pmid26056137      J+Cell+Biol 2015 ; 209 (5): 633-44
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  • Nanobodies and recombinant binders in cell biology #MMPMID26056137
  • Helma J; Cardoso MC; Muyldermans S; Leonhardt H
  • J Cell Biol 2015[Jun]; 209 (5): 633-44 PMID26056137show ga
  • Antibodies are key reagents to investigate cellular processes. The development of recombinant antibodies and binders derived from natural protein scaffolds has expanded traditional applications, such as immunofluorescence, binding arrays, and immunoprecipitation. In addition, their small size and high stability in ectopic environments have enabled their use in all areas of cell research, including structural biology, advanced microscopy, and intracellular expression. Understanding these novel reagents as genetic modules that can be integrated into cellular pathways opens up a broad experimental spectrum to monitor and manipulate cellular processes.
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