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10.1083/jcb.201409133 http://scihub22266oqcxt.onion/10.1083/jcb.201409133 C4460149!4460149 !26056140     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26056140 &cmd=llinks): Failed to open stream: HTTP request failed! 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Live imaging and modeling of inner nuclear membrane targeting reveals its molecular requirements in mammalian cells |pdf=|usr=}}{{26056140 }} gab.com Text Live imaging and modeling of inner nuclear membrane targeting reveals its molecular requirements in mammalian cells JO: J Cell Biol http://www.kidney.de/pget.php?&tw=1&pmid=26056140 #FOAvip Targeting of inner nuclear membrane (INM) proteins is essential for nuclear architecture and function, yet its mechanism remains poorly understood. Here, we established a new reporter that allows real-time imaging of membrane protein transport from the ER to the INM using Lamin B receptor and Lap2? as model INM proteins. These reporters allowed us to characterize the kinetics of INM targeting and establish a mathematical model of this process and enabled us to probe its molecular requirements in an RNA interference screen of 96 candidate genes. Modeling of the phenotypes of genes involved in transport of these INM proteins predicted that it critically depended on the number and permeability of nuclear pores and the availability of nuclear binding sites, but was unaffected by depletion of most transport receptors. These predictions were confirmed with targeted validation experiments on the functional requirements of nucleoporins and nuclear lamins. Collectively, our data support a diffusion retention model of INM protein transport in mammalian cells. Twit Text FOAVip Live imaging and modeling of inner nuclear membrane targeting reveals its molecular requirements in mammalian cells ????J Cell Biol http://www.kidney.de/pget.php?&tw=1&pmid=26056140 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26056140 #FOAvip English Wikipedia <ref>{{Cite pmid|26056140 }}</ref> Live imaging and modeling of inner nuclear membrane targeting reveals its molecular requirements in mammalian cells #MMPMID26056140 Boni A ; Politi AZ ; Strnad P ; Xiang W ; Hossain MJ ; Ellenberg J J Cell Biol 2015[Jun]; 209 (5 ): 705-20 PMID26056140 show ga Targeting of inner nuclear membrane (INM) proteins is essential for nuclear architecture and function, yet its mechanism remains poorly understood. Here, we established a new reporter that allows real-time imaging of membrane protein transport from the ER to the INM using Lamin B receptor and Lap2? as model INM proteins. These reporters allowed us to characterize the kinetics of INM targeting and establish a mathematical model of this process and enabled us to probe its molecular requirements in an RNA interference screen of 96 candidate genes. Modeling of the phenotypes of genes involved in transport of these INM proteins predicted that it critically depended on the number and permeability of nuclear pores and the availability of nuclear binding sites, but was unaffected by depletion of most transport receptors. These predictions were confirmed with targeted validation experiments on the functional requirements of nucleoporins and nuclear lamins. Collectively, our data support a diffusion retention model of INM protein transport in mammalian cells. |*Models, Biological [MESH] |*Molecular Imaging [MESH] |Active Transport, Cell Nucleus/physiology [MESH] |DNA-Binding Proteins/genetics/*metabolism [MESH] |Endoplasmic Reticulum/genetics/*metabolism [MESH] |HeLa Cells [MESH] |Humans [MESH] |Lamin B Receptor [MESH] |Membrane Proteins/genetics/*metabolism [MESH] |Nuclear Envelope/genetics/*metabolism [MESH]Live imaging and modeling of inner nuclear membrane targeting reveals (epmc).pdf DeepDyve Pubget Overpricing