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10.1007/978-1-62703-845-4_16 http://scihub22266oqcxt.onion/10.1007/978-1-62703-845-4_16 C4459639!4459639!24504957     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Methods+Mol+Biol 2014 ; 1124 (ä): 251-68 Nephropedia Template TP {{tp|p=24504957|t=2014. Immunofluorescence and Confocal Microscopy of Neutrophils |pdf=|usr=}}{{24504957}} gab.com Text Immunofluorescence and Confocal Microscopy of Neutrophils JO: Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=24504957 #FOAvip Rapid recruitment of neutrophils to sites of infection and their ability to phagocytose and kill microbes is an important aspect of the innate immune response. Challenges associated with imaging of these cells include their short lifespan and small size and the fact that unstimulated cells are nonadherent. In addition, although cytoplasmic granules are plentiful, the abundance of many other organelles is diminished. Here we reprise methods for analysis of resting and activated cells using immunofluorescence and confocal microscopy, including kinetic analysis of phagosome maturation and degranulation, and detection of intraphagosomal superoxide accumulation. We describe approaches for rapid cell fixation and permeabilization that maximize antigen detection and discuss other variables that also affect data interpretation and image quality (such as cell spreading, degranulation, and phagocytosis). Finally, we show that these methods are also applicable to studies of neutrophil interactions with the extracellular matrix. Twit Text FOAVip Immunofluorescence and Confocal Microscopy of Neutrophils ????Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=24504957 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24504957 #FOAvip English Wikipedia <ref>{{Cite pmid|24504957}}</ref> Immunofluorescence and Confocal Microscopy of Neutrophils #MMPMID24504957 Allen LAH Methods Mol Biol 2014[]; 1124 (ä): 251-68 PMID24504957show ga Rapid recruitment of neutrophils to sites of infection and their ability to phagocytose and kill microbes is an important aspect of the innate immune response. Challenges associated with imaging of these cells include their short lifespan and small size and the fact that unstimulated cells are nonadherent. In addition, although cytoplasmic granules are plentiful, the abundance of many other organelles is diminished. Here we reprise methods for analysis of resting and activated cells using immunofluorescence and confocal microscopy, including kinetic analysis of phagosome maturation and degranulation, and detection of intraphagosomal superoxide accumulation. We describe approaches for rapid cell fixation and permeabilization that maximize antigen detection and discuss other variables that also affect data interpretation and image quality (such as cell spreading, degranulation, and phagocytosis). Finally, we show that these methods are also applicable to studies of neutrophil interactions with the extracellular matrix. äImmunofluorescence and Confocal Microscopy of Neutrophils (epmc).pdf DeepDyve Pubget Overpricing