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10.1111/mmi.12703 http://scihub22266oqcxt.onion/10.1111/mmi.12703 C4459576!4459576!24995493     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Microbiol 2014 ; 93 (5): 883-96 Nephropedia Template TP {{tp|p=24995493|t=2014. De novo morphogenesis in L-forms via geometric control of cell growth |pdf=|usr=}}{{24995493}} gab.com Text De novo morphogenesis in L-forms via geometric control of cell growth JO: Mol Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=24995493 #FOAvip In virtually all bacteria, the cell wall is crucial for mechanical integrity and for determining cell shape. Escherichia coli?s rod-like shape is maintained via the spatiotemporal patterning of cell-wall synthesis by the actin homologue MreB. Here, we transiently inhibited cell-wall synthesis in E. coli to generate cell-wall-deficient, spherical L-forms, and found that they robustly reverted to a rod-like shape within several generations after inhibition cessation. The chemical composition of the cell wall remained essentially unchanged during this process, as indicated by liquid chromatography. Throughout reversion, MreB localized to inwardly curved regions of the cell, and fluorescent cell wall labelling revealed that MreB targets synthesistothose regions. When exposed to the MreB inhibitor A22, reverting cells regrew a cell wall but failed to recover a rod-like shape. Our results suggest that MreB provides the geometric measure that allows E. coli to actively establish and regulate its morphology. Twit Text FOAVip De novo morphogenesis in L-forms via geometric control of cell growth ????Mol Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=24995493 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=24995493 #FOAvip English Wikipedia <ref>{{Cite pmid|24995493}}</ref> De novo morphogenesis in L-forms via geometric control of cell growth #MMPMID24995493 Billings G; Ouzounov N; Ursell T; Desmarais SM; Shaevitz J; Gitai Z; Huang KC Mol Microbiol 2014[Sep]; 93 (5): 883-96 PMID24995493show ga In virtually all bacteria, the cell wall is crucial for mechanical integrity and for determining cell shape. Escherichia coli?s rod-like shape is maintained via the spatiotemporal patterning of cell-wall synthesis by the actin homologue MreB. Here, we transiently inhibited cell-wall synthesis in E. coli to generate cell-wall-deficient, spherical L-forms, and found that they robustly reverted to a rod-like shape within several generations after inhibition cessation. The chemical composition of the cell wall remained essentially unchanged during this process, as indicated by liquid chromatography. Throughout reversion, MreB localized to inwardly curved regions of the cell, and fluorescent cell wall labelling revealed that MreB targets synthesistothose regions. When exposed to the MreB inhibitor A22, reverting cells regrew a cell wall but failed to recover a rod-like shape. Our results suggest that MreB provides the geometric measure that allows E. coli to actively establish and regulate its morphology. äDe novo morphogenesis in L-forms via geometric control of cell growth (epmc).pdf DeepDyve Pubget Overpricing