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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 J+Hematol+Oncol
2015 ; 8
(ä): 56
Nephropedia Template TP
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microRNA-26a suppresses recruitment of macrophages by down-regulating macrophage
colony-stimulating factor expression through the PI3K/Akt pathway in
hepatocellular carcinoma
#MMPMID26021873
Chai ZT
; Zhu XD
; Ao JY
; Wang WQ
; Gao DM
; Kong J
; Zhang N
; Zhang YY
; Ye BG
; Ma DN
; Cai H
; Sun HC
J Hematol Oncol
2015[May]; 8
(ä): 56
PMID26021873
show ga
BACKGROUND: microRNAs (miRNAs) have been reported to modulate macrophage
colony-stimulating factor (M-CSF) and macrophages. The aim of this study was to
find whether miR-26a can suppress M-CSF expression and the recruitment of
macrophages. METHODS: Hepatocellular carcinoma (HCC) cell lines with decreased or
increased expression of miR-26a were established in a previous study. M-CSF
expression by tumor cells was measured by enzyme-linked immunosorbent assay, and
cell migration assays were used to explore the effect of HCC cell lines on
macrophage recruitment in vitro. Real-time PCR measured a panel of mRNAs
expressed by macrophages. Xenograft models were used to observe tumor growth.
Immunohistochemistry was conducted to study the relation between miR-26a
expression and M-CSF expression and macrophage recruitment in patients with HCC.
RESULTS: Ectopic expression of miR-26a reduced expression of M-CSF. The
conditioned medium (CM) from HepG2 cells that overexpressed miR-26a reduced the
migration ability of THP-1 cells stimulated by phorbol myristate acetate (PMA)
increased expression of interleukin (IL)-12b or IL-23 mRNA and decreased
expression of chemokine (C-C motif) ligand (CCL)22, CCL17, and IL-10 mRNA, in
comparison to the medium from the parental HepG2 cells. These effects could be
interrupted by the PI3K/Akt pathway inhibitor LY294002. Ectopic expression of
miR-26a in HCC cells suppressed tumor growth, M-CSF expression, and infiltration
of macrophages in tumors. Similar results were also found when using HCCLM3
cells. Furthermore, the expression of miR-26a was inversely correlated with M-CSF
expression and macrophage infiltration in tumor tissues from patients with HCC.
CONCLUSIONS: miR-26a expression reduced M-CSF expression and recruitment of
macrophages in HCC.