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10.1038/bonekey.2015.66

http://scihub22266oqcxt.onion/10.1038/bonekey.2015.66
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suck abstract from ncbi


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pmid26131356      Bonekey+Rep 2015 ; 4 (ä): ä
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  • Alteration of Type I collagen microstructure induced by estrogen depletion can be prevented with drug treatment #MMPMID26131356
  • Cauble MA; Rothman E; Welch K; Fang M; Duong LT; Pennypacker BL; Orr BG; Banaszak Holl MM
  • Bonekey Rep 2015[]; 4 (ä): ä PMID26131356show ga
  • Two independent biological replicates of estrogen depletion were employed with differing drug treatment conditions. Data Set I consisted of 9-month-old New Zealand white female rabbits treated as follows: sham-operated (n=11), ovariectomized (OVX; n=12), OVX+200??g?kg?1 alendronate (ALN), 3 × a week for 27 weeks (n=12) and OVX+10?mg?kg?1 Cathepsin-K inhibitor (CatKI) daily for 27 weeks. Data Set II consisted of 6-month-old New Zealand white female rabbits that were sham-operated (n=12), OVX (n=12) or OVX+0.05?mg?kg?1 17?-estradiol (ERT) 3 × a week for 13 weeks (n=12). Samples from the cortical femur were polished and demineralized to make them suitable for atomic force microscopy (AFM) imaging. Type I collagen fibrils present in bundles or sheets, running parallel to each other, were combined into a class termed Parallel. Fibrils present outside of such structures, typically in images with an angular range of non-parallel fibrils, were combined into a class termed Oblique. The percentage of fibrils coded as Parallel for Sham animals in Data Sets I and II was 52% and 53%, respectively. The percentage of fibrils coded as Parallel for OVX animals in Data Sets I and II was 35% in both cases. ALN and ERT drug treatments reduced the change from 18 to 12%, whereas CatKI treatment reduced the change to 5%.
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