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10.3390/ijerph120504921 http://scihub22266oqcxt.onion/10.3390/ijerph120504921 C4454946!4454946!25955528     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Int+J+Environ+Res+Public+Health 2015 ; 12 (5): 4921-41 Nephropedia Template TP {{tp|p=25955528|t=2015. Molecular Detection of a Potentially Toxic Diatom Species |pdf=|usr=}}{{25955528}} gab.com Text Molecular Detection of a Potentially Toxic Diatom Species JO: Int J Environ Res Public Health http://www.kidney.de/pget.php?&tw=1&pmid=25955528 #FOAvip A few diatom species produce toxins that affect human and animal health. Among these, members of the Pseudo-nitzschia genus were the first diatoms unambiguously identified as producer of domoic acid, a neurotoxin affecting molluscan shell-fish, birds, marine mammals, and humans. Evidence exists indicating the involvement of another diatom genus, Amphora, as a potential producer of domoic acid. We present a strategy for the detection of the diatom species Amphora coffeaeformis based on the development of species-specific oligonucleotide probes and their application in microarray hybridization experiments. This approach is based on the use of two marker genes highly conserved in all diatoms, but endowed with sufficient genetic divergence to discriminate diatoms at the species level. A region of approximately 450 bp of these previously unexplored marker genes, coding for elongation factor 1-a (eEF1-a) and silicic acid transporter (SIT), was used to design oligonucleotide probes that were tested for specificity in combination with the corresponding fluorescently labeled DNA targets. The results presented in this work suggest a possible use of this DNA chip technology for the selective detection of A. coffeaeformis in environmental settings where the presence of this potential toxin producer may represent a threat to human and animal health. In addition, the same basic approach can be adapted to a wider range of diatoms for the simultaneous detection of microorganisms used as biomarkers of different water quality levels. Twit Text FOAVip Molecular Detection of a Potentially Toxic Diatom Species ????Int J Environ Res Public Health http://www.kidney.de/pget.php?&tw=1&pmid=25955528 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25955528 #FOAvip English Wikipedia <ref>{{Cite pmid|25955528}}</ref> Molecular Detection of a Potentially Toxic Diatom Species #MMPMID25955528 Dhar BC; Cimarelli L; Singh KS; Brandi L; Brandi A; Puccinelli C; Marcheggiani S; Spurio R Int J Environ Res Public Health 2015[May]; 12 (5): 4921-41 PMID25955528show ga A few diatom species produce toxins that affect human and animal health. Among these, members of the Pseudo-nitzschia genus were the first diatoms unambiguously identified as producer of domoic acid, a neurotoxin affecting molluscan shell-fish, birds, marine mammals, and humans. Evidence exists indicating the involvement of another diatom genus, Amphora, as a potential producer of domoic acid. We present a strategy for the detection of the diatom species Amphora coffeaeformis based on the development of species-specific oligonucleotide probes and their application in microarray hybridization experiments. This approach is based on the use of two marker genes highly conserved in all diatoms, but endowed with sufficient genetic divergence to discriminate diatoms at the species level. A region of approximately 450 bp of these previously unexplored marker genes, coding for elongation factor 1-a (eEF1-a) and silicic acid transporter (SIT), was used to design oligonucleotide probes that were tested for specificity in combination with the corresponding fluorescently labeled DNA targets. The results presented in this work suggest a possible use of this DNA chip technology for the selective detection of A. coffeaeformis in environmental settings where the presence of this potential toxin producer may represent a threat to human and animal health. In addition, the same basic approach can be adapted to a wider range of diatoms for the simultaneous detection of microorganisms used as biomarkers of different water quality levels. äMolecular Detection of a Potentially Toxic Diatom Species (epmc).pdf DeepDyve Pubget Overpricing