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2015 ; 87
(4
): 289-92
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gab.com Text
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OMIP-025: evaluation of human T- and NK-cell responses including memory and
follicular helper phenotype by intracellular cytokine staining
#MMPMID25407958
Moncunill G
; Dobaņo C
; McElrath MJ
; De Rosa SC
Cytometry A
2015[Apr]; 87
(4
): 289-92
PMID25407958
show ga
This panel was developed to assess antigen-specific T cells using peptide pools
to various antigens of interest, although other types of antigens such as
recombinant proteins or whole pathogens could be considered using different
stimulation times. In addition to multiple functional markers, the panel includes
differentiation markers and markers to assess follicular helper T cells and NK
cells (Table 1). It was optimized using cryopreserved peripheral blood
mononuclear cells (PBMC) from human immunodeficiency virus (HIV) uninfected and
HIV infected adults with known cytomegalovirus (CMV) responses and it underwent
assay qualification. The panel is being used to evaluate the responses to HIV and
malaria vaccine candidates in adults and children from different geographic
areas.