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10.1002/cyto.a.22590

http://scihub22266oqcxt.onion/10.1002/cyto.a.22590
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C4454451!4454451 !25407958
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suck abstract from ncbi


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pmid25407958
      Cytometry+A 2015 ; 87 (4 ): 289-92
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  • OMIP-025: evaluation of human T- and NK-cell responses including memory and follicular helper phenotype by intracellular cytokine staining #MMPMID25407958
  • Moncunill G ; Dobaņo C ; McElrath MJ ; De Rosa SC
  • Cytometry A 2015[Apr]; 87 (4 ): 289-92 PMID25407958 show ga
  • This panel was developed to assess antigen-specific T cells using peptide pools to various antigens of interest, although other types of antigens such as recombinant proteins or whole pathogens could be considered using different stimulation times. In addition to multiple functional markers, the panel includes differentiation markers and markers to assess follicular helper T cells and NK cells (Table 1). It was optimized using cryopreserved peripheral blood mononuclear cells (PBMC) from human immunodeficiency virus (HIV) uninfected and HIV infected adults with known cytomegalovirus (CMV) responses and it underwent assay qualification. The panel is being used to evaluate the responses to HIV and malaria vaccine candidates in adults and children from different geographic areas.
  • |AIDS Vaccines/immunology [MESH]
  • |Adult [MESH]
  • |Antigens, CD/immunology [MESH]
  • |Biomarkers/analysis [MESH]
  • |Child [MESH]
  • |Cytokines/*analysis [MESH]
  • |Cytomegalovirus/immunology [MESH]
  • |Flow Cytometry/*methods [MESH]
  • |HIV Infections/immunology [MESH]
  • |Humans [MESH]
  • |Immunologic Memory/immunology [MESH]
  • |Interferon-gamma/immunology [MESH]
  • |Interleukin-2/immunology [MESH]
  • |Killer Cells, Natural/*immunology [MESH]
  • |Leukocytes, Mononuclear/immunology [MESH]
  • |Malaria Vaccines/immunology [MESH]
  • |Phenotype [MESH]
  • |Staining and Labeling/*methods [MESH]
  • |T-Lymphocytes, Helper-Inducer/*immunology [MESH]


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