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10.1038/bjc.2014.425 http://scihub22266oqcxt.onion/10.1038/bjc.2014.425 C4453854!4453854 !25072259     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25072259 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Br+J+Cancer 2014 ; 111 (6 ): 1159-67 Nephropedia Template TP {{tp|p=25072259 |t=2014. Glutamine depletion by crisantaspase hinders the growth of human hepatocellular carcinoma xenografts |pdf=|usr=}}{{25072259 }} gab.com Text Glutamine depletion by crisantaspase hinders the growth of human hepatocellular carcinoma xenografts JO: Br J Cancer http://www.kidney.de/pget.php?&tw=1&pmid=25072259 #FOAvip BACKGROUND: A subset of human hepatocellular carcinomas (HCC) exhibit mutations of ?-catenin gene CTNNB1 and overexpress Glutamine synthetase (GS). The CTNNB1-mutated HCC cell line HepG2 is sensitive to glutamine starvation induced in vitro with the antileukemic drug Crisantaspase and the GS inhibitor methionine-L-sulfoximine (MSO). METHODS: Immunodeficient mice with subcutaneous xenografts of the CTNNB1-mutated HCC cell lines HepG2 and HC-AFW1 were treated with Crisantaspase and/or MSO, and tumour growth was monitored. At the end of treatment, tumour weight and histology were assessed. Serum and tissue amino acids were determined by HPLC. Gene and protein expression were estimated with RT-PCR and western blot and GS activity with a colorimetric method. mTOR activity was evaluated from the phosphorylation of p70S6K1. RESULTS: Crisantaspase and MSO depleted serum glutamine, lowered glutamine in liver and tumour tissue, and inhibited liver GS activity. HepG2 tumour growth was significantly reduced by either Crisantaspase or MSO, and completely suppressed by the combined treatment. The combined treatment was also effective against xenografts of the HC-AFW1 cell line, which is Crisantaspase resistant in vitro. CONCLUSIONS: The combination of Crisantaspase and MSO reduces glutamine supply to CTNNB1-mutated HCC xenografts and hinders their growth. Twit Text FOAVip Glutamine depletion by crisantaspase hinders the growth of human hepatocellular carcinoma xenografts ????Br J Cancer http://www.kidney.de/pget.php?&tw=1&pmid=25072259 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25072259 #FOAvip English Wikipedia <ref>{{Cite pmid|25072259 }}</ref> Glutamine depletion by crisantaspase hinders the growth of human hepatocellular carcinoma xenografts #MMPMID25072259 Chiu M ; Tardito S ; Pillozzi S ; Arcangeli A ; Armento A ; Uggeri J ; Missale G ; Bianchi MG ; Barilli A ; Dall'Asta V ; Campanini N ; Silini EM ; Fuchs J ; Armeanu-Ebinger S ; Bussolati O Br J Cancer 2014[Sep]; 111 (6 ): 1159-67 PMID25072259 show ga BACKGROUND: A subset of human hepatocellular carcinomas (HCC) exhibit mutations of ?-catenin gene CTNNB1 and overexpress Glutamine synthetase (GS). The CTNNB1-mutated HCC cell line HepG2 is sensitive to glutamine starvation induced in vitro with the antileukemic drug Crisantaspase and the GS inhibitor methionine-L-sulfoximine (MSO). METHODS: Immunodeficient mice with subcutaneous xenografts of the CTNNB1-mutated HCC cell lines HepG2 and HC-AFW1 were treated with Crisantaspase and/or MSO, and tumour growth was monitored. At the end of treatment, tumour weight and histology were assessed. Serum and tissue amino acids were determined by HPLC. Gene and protein expression were estimated with RT-PCR and western blot and GS activity with a colorimetric method. mTOR activity was evaluated from the phosphorylation of p70S6K1. RESULTS: Crisantaspase and MSO depleted serum glutamine, lowered glutamine in liver and tumour tissue, and inhibited liver GS activity. HepG2 tumour growth was significantly reduced by either Crisantaspase or MSO, and completely suppressed by the combined treatment. The combined treatment was also effective against xenografts of the HC-AFW1 cell line, which is Crisantaspase resistant in vitro. CONCLUSIONS: The combination of Crisantaspase and MSO reduces glutamine supply to CTNNB1-mutated HCC xenografts and hinders their growth. |*Glutamine/analysis/blood [MESH] |Animals [MESH] |Antineoplastic Agents/therapeutic use [MESH] |Asparaginase/*pharmacology/*therapeutic use [MESH] |Asparagine/blood [MESH] |Cadherins/analysis [MESH] |Carcinoma, Hepatocellular/*drug therapy/enzymology/genetics/pathology [MESH] |Enzyme Inhibitors/therapeutic use [MESH] |Glutamate-Ammonia Ligase/*antagonists & inhibitors/genetics/metabolism [MESH] |Hep G2 Cells [MESH] |Humans [MESH] |Ki-67 Antigen/analysis [MESH] |Liver Neoplasms/*drug therapy/enzymology/genetics/pathology [MESH] |Male [MESH] |Methionine Sulfoximine/therapeutic use [MESH] |Mice [MESH] |Mice, Nude [MESH] |Mutation [MESH] |Tumor Burden/*drug effects [MESH] |Xenograft Model Antitumor Assays [MESH]Glutamine depletion by crisantaspase hinders the growth of human hepat(epmc).pdf DeepDyve Pubget Overpricing