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10.1186/s12891-015-0606-2 http://scihub22266oqcxt.onion/10.1186/s12891-015-0606-2 C4453221!4453221!26037065     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       BMC+Musculoskelet+Disord 2015 ; 16 (ä): ä Nephropedia Template TP {{tp|p=26037065|t=2015. Apoptosis associated with Wnt/?-catenin pathway leads to steroid-induced avascular necrosis of femoral head |pdf=|usr=}}{{26037065}} gab.com Text Apoptosis associated with Wnt/ -catenin pathway leads to steroid-induced avascular necrosis of femoral head JO: BMC Musculoskelet Disord http://www.kidney.de/pget.php?&tw=1&pmid=26037065 #FOAvip Background: The objective of the current study was to establish a rat model to investigate apoptosis in steroid-induced femoral head osteonecrosis occurring via the Wnt/?-catenin pathway. Methods: Male Sprague?Dawley (SD) rats were randomly divided into a control group (group A), model group (group B) and sFRP1 group (group C), each consisting of 24 rats, and the rats were intravenously injected with LPS (10 ?g/kg body weight). After 24 h, three injections of MPS (20 mg/kg body weight) were administered intramuscularly at 24-h intervals. The rats in group C were injected intramuscularly with 1 ?g/kg sFRP1 protein per day for 30 days, beginning at the time of the first MPS administration. The group A rats were fed and housed under identical conditions but received saline injection. All animals were sacrificed at weeks 2, 4 and 8 from the first MPS injection. Histopathological staining was preformed to evaluated osteonecrosis. Apoptosis was detected via quantitative terminal deoxynucleotidyl transferase (TdT) deoxyuridine triphosphate nick-end labelling (TUNEL) staining, caspase-3 activity assay, and detection of Bcl-2 and Bax protein expression by immunohistochemistry and Western blotting. Wnt/?-catenin pathway signalling molecules, including activated ?-catenin and c-Myc, were detected by immunohistochemistry and Western blotting. Results: Typical osteonecrosis was observed in groups B and C. Apoptosis gradually increased with increasing time in both groups B and C. More severe osteonecrosis and apoptosis were observed in group C compared with group B. The expression levels of caspase-3 and Bax were higher while that of Bcl-2 was lower in group C compared with group B. The expression levels of activated ?-catenin and c-Myc gradually decreased with increasing time in both groups B and C, and they were lower in group C compared with group B. Conclusions: The Wnt/?-catenin pathway is involved in the pathogenesis of early stage SANFH, as we have demonstrated in an SANFH rat model, and it may act through the regulation of c-Myc, which affects the cell cycle and cell apoptosis. Electronic supplementary material: The online version of this article (doi:10.1186/s12891-015-0606-2) contains supplementary material, which is available to authorized users. Twit Text FOAVip Apoptosis associated with Wnt/ -catenin pathway leads to steroid-induced avascular necrosis of femoral head ????BMC Musculoskelet Disord http://www.kidney.de/pget.php?&tw=1&pmid=26037065 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26037065 #FOAvip English Wikipedia <ref>{{Cite pmid|26037065}}</ref> Apoptosis associated with Wnt/?-catenin pathway leads to steroid-induced avascular necrosis of femoral head #MMPMID26037065 Zhang C; Zou Yl; Ma J; Dang Xq; Wang Kz BMC Musculoskelet Disord 2015[]; 16 (ä): ä PMID26037065show ga Background: The objective of the current study was to establish a rat model to investigate apoptosis in steroid-induced femoral head osteonecrosis occurring via the Wnt/?-catenin pathway. Methods: Male Sprague?Dawley (SD) rats were randomly divided into a control group (group A), model group (group B) and sFRP1 group (group C), each consisting of 24 rats, and the rats were intravenously injected with LPS (10 ?g/kg body weight). After 24 h, three injections of MPS (20 mg/kg body weight) were administered intramuscularly at 24-h intervals. The rats in group C were injected intramuscularly with 1 ?g/kg sFRP1 protein per day for 30 days, beginning at the time of the first MPS administration. The group A rats were fed and housed under identical conditions but received saline injection. All animals were sacrificed at weeks 2, 4 and 8 from the first MPS injection. Histopathological staining was preformed to evaluated osteonecrosis. Apoptosis was detected via quantitative terminal deoxynucleotidyl transferase (TdT) deoxyuridine triphosphate nick-end labelling (TUNEL) staining, caspase-3 activity assay, and detection of Bcl-2 and Bax protein expression by immunohistochemistry and Western blotting. Wnt/?-catenin pathway signalling molecules, including activated ?-catenin and c-Myc, were detected by immunohistochemistry and Western blotting. Results: Typical osteonecrosis was observed in groups B and C. Apoptosis gradually increased with increasing time in both groups B and C. More severe osteonecrosis and apoptosis were observed in group C compared with group B. The expression levels of caspase-3 and Bax were higher while that of Bcl-2 was lower in group C compared with group B. The expression levels of activated ?-catenin and c-Myc gradually decreased with increasing time in both groups B and C, and they were lower in group C compared with group B. Conclusions: The Wnt/?-catenin pathway is involved in the pathogenesis of early stage SANFH, as we have demonstrated in an SANFH rat model, and it may act through the regulation of c-Myc, which affects the cell cycle and cell apoptosis. Electronic supplementary material: The online version of this article (doi:10.1186/s12891-015-0606-2) contains supplementary material, which is available to authorized users. äApoptosis associated with Wnt/?-catenin pathway leads to steroid-induc(epmc).pdf DeepDyve Pubget Overpricing