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Effect of Leflunomide on the Abnormal Expression of Lipid Rafts and F-Actin in B
Lymphocytes from Patients with Systemic Lupus Erythematosus
#MMPMID26090500
Dong GF
; Zhang X
; He DN
; Li L
; Zhang GF
J Immunol Res
2015[]; 2015
(?): 832916
PMID26090500
show ga
PURPOSES: To investigate the possible changes in B cell subsets and in B cell
expression patterns of lipid rafts (LRs) and F-actin in patients with SLE and
whether leflunomide treatment may have effect on these changes. METHODS: The B
cell subsets and LRs expression were determined by flow cytometry and confocal
microscopy, and F-actin expression was examined by confocal microscopy. RESULTS:
CD27(+)IgD(+) B cell subsets were significantly decreased while CD38(+)CD95(+) B
cell subsets increased in SLE patients. The LRs levels of B cells were remarkably
increased and positively correlated with SLEDAI and anti-dsDNA titer in SLE
patients. The expression level of LRs was significantly higher in CD38(+) B cells
than CD38(-) B cells and negatively correlated with C3 levels. The increased
expression of LRs was associated with reduced expression of F-actin in the B
cells from active SLE patients. Furthermore, in vitro treatment of the cells with
A771726 reduced the expression level of LRs, attenuated the overaggregation of
LRs, and normalized the distribution of F-actin. CONCLUSIONS: There were
abnormalities in B cell subsets and LRs and F-actin expression of B cell from SLE
patients. Modulation of B cell expression of LRs and F-actin by LEF could be a
potential therapeutic target for SLE.
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