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10.3389/fcimb.2015.00048 http://scihub22266oqcxt.onion/10.3389/fcimb.2015.00048 C4451638!4451638!26082896     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Front+Cell+Infect+Microbiol 2015 ; 5 (ä): ä Nephropedia Template TP {{tp|p=26082896|t=2015. Purification and proteomics of pathogen-modified vacuoles and membranes |pdf=|usr=}}{{26082896}} gab.com Text Purification and proteomics of pathogen-modified vacuoles and membranes JO: Front Cell Infect Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=26082896 #FOAvip Certain pathogenic bacteria adopt an intracellular lifestyle and proliferate in eukaryotic host cells. The intracellular niche protects the bacteria from cellular and humoral components of the mammalian immune system, and at the same time, allows the bacteria to gain access to otherwise restricted nutrient sources. Yet, intracellular protection and access to nutrients comes with a price, i.e., the bacteria need to overcome cell-autonomous defense mechanisms, such as the bactericidal endocytic pathway. While a few bacteria rupture the early phagosome and escape into the host cytoplasm, most intracellular pathogens form a distinct, degradation-resistant and replication-permissive membranous compartment. Intracellular bacteria that form unique pathogen vacuoles include Legionella, Mycobacterium, Chlamydia, Simkania, and Salmonella species. In order to understand the formation of these pathogen niches on a global scale and in a comprehensive and quantitative manner, an inventory of compartment-associated host factors is required. To this end, the intact pathogen compartments need to be isolated, purified and biochemically characterized. Here, we review recent progress on the isolation and purification of pathogen-modified vacuoles and membranes, as well as their proteomic characterization by mass spectrometry and different validation approaches. These studies provide the basis for further investigations on the specific mechanisms of pathogen-driven compartment formation. Twit Text FOAVip Purification and proteomics of pathogen-modified vacuoles and membranes ????Front Cell Infect Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=26082896 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26082896 #FOAvip English Wikipedia <ref>{{Cite pmid|26082896}}</ref> Purification and proteomics of pathogen-modified vacuoles and membranes #MMPMID26082896 Herweg JA; Hansmeier N; Otto A; Geffken AC; Subbarayal P; Prusty BK; Becher D; Hensel M; Schaible UE; Rudel T; Hilbi H Front Cell Infect Microbiol 2015[]; 5 (ä): ä PMID26082896show ga Certain pathogenic bacteria adopt an intracellular lifestyle and proliferate in eukaryotic host cells. The intracellular niche protects the bacteria from cellular and humoral components of the mammalian immune system, and at the same time, allows the bacteria to gain access to otherwise restricted nutrient sources. Yet, intracellular protection and access to nutrients comes with a price, i.e., the bacteria need to overcome cell-autonomous defense mechanisms, such as the bactericidal endocytic pathway. While a few bacteria rupture the early phagosome and escape into the host cytoplasm, most intracellular pathogens form a distinct, degradation-resistant and replication-permissive membranous compartment. Intracellular bacteria that form unique pathogen vacuoles include Legionella, Mycobacterium, Chlamydia, Simkania, and Salmonella species. In order to understand the formation of these pathogen niches on a global scale and in a comprehensive and quantitative manner, an inventory of compartment-associated host factors is required. To this end, the intact pathogen compartments need to be isolated, purified and biochemically characterized. Here, we review recent progress on the isolation and purification of pathogen-modified vacuoles and membranes, as well as their proteomic characterization by mass spectrometry and different validation approaches. These studies provide the basis for further investigations on the specific mechanisms of pathogen-driven compartment formation. äPurification and proteomics of pathogen-modified vacuoles and membrane(epmc).pdf DeepDyve Pubget Overpricing