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10.1186/s12977-015-0174-4 http://scihub22266oqcxt.onion/10.1186/s12977-015-0174-4 C4450836!4450836!26032178     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Retrovirology 2015 ; 12 (ä): ä Nephropedia Template TP {{tp|p=26032178|t=2015. SAMHD1 specifically restricts retroviruses through its RNase activity |pdf=|usr=}}{{26032178}} gab.com Text SAMHD1 specifically restricts retroviruses through its RNase activity JO: Retrovirology http://www.kidney.de/pget.php?&tw=1&pmid=26032178 #FOAvip Background: Human SAMHD1 possesses dual enzymatic functions. It acts as both a dGTP-dependent triphosphohydrolase and as an exoribonuclease. The dNTPase function depletes the cellular dNTP pool, which is required for retroviral reverse transcription in differentiated myeloid cells and resting CD4+ T cells; thus this activity mainly plays a role in SAMHD1-mediated retroviral restriction. However, a recent study demonstrated that SAMHD1 directly targets HIV-1 genomic RNA via its RNase activity, and that this function (rather than dNTPase activity) is sufficient for HIV-1 restriction. While HIV-1 genomic RNA is a potent target for SAMHD1 during viral infection, the specificity of SAMHD1-mediated RNase activity during infection by other viruses is unclear. Results: The results of the present study showed that SAMHD1 specifically degrades retroviral genomic RNA in monocyte-derived macrophage-like cells and in primary monocyte-derived macrophages. Consistent with this, SAMHD1 selectively restricted retroviral replication, but did not affect the replication of other common non-retro RNA genome viruses, suggesting that the RNase-mediated antiviral function of SAMHD1 is limited to retroviruses. In addition, neither inhibiting reverse transcription by treatment with several reverse transcriptase inhibitors nor infection with reverse transcriptase-defective HIV-1 altered RNA levels after viral challenge, indicating that the retrovirus-specific RNase function is not dependent on processes associated with retroviral reverse transcription. Conclusions: The results presented herein suggest that the RNase activity of SAMHD1 is sufficient to control the replication of retroviruses, but not that of non-retro RNA viruses. Electronic supplementary material: The online version of this article (doi:10.1186/s12977-015-0174-4) contains supplementary material, which is available to authorized users. Twit Text FOAVip SAMHD1 specifically restricts retroviruses through its RNase activity ????Retrovirology http://www.kidney.de/pget.php?&tw=1&pmid=26032178 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26032178 #FOAvip English Wikipedia <ref>{{Cite pmid|26032178}}</ref> SAMHD1 specifically restricts retroviruses through its RNase activity #MMPMID26032178 Choi J; Ryoo J; Oh C; Hwang S; Ahn K Retrovirology 2015[]; 12 (ä): ä PMID26032178show ga Background: Human SAMHD1 possesses dual enzymatic functions. It acts as both a dGTP-dependent triphosphohydrolase and as an exoribonuclease. The dNTPase function depletes the cellular dNTP pool, which is required for retroviral reverse transcription in differentiated myeloid cells and resting CD4+ T cells; thus this activity mainly plays a role in SAMHD1-mediated retroviral restriction. However, a recent study demonstrated that SAMHD1 directly targets HIV-1 genomic RNA via its RNase activity, and that this function (rather than dNTPase activity) is sufficient for HIV-1 restriction. While HIV-1 genomic RNA is a potent target for SAMHD1 during viral infection, the specificity of SAMHD1-mediated RNase activity during infection by other viruses is unclear. Results: The results of the present study showed that SAMHD1 specifically degrades retroviral genomic RNA in monocyte-derived macrophage-like cells and in primary monocyte-derived macrophages. Consistent with this, SAMHD1 selectively restricted retroviral replication, but did not affect the replication of other common non-retro RNA genome viruses, suggesting that the RNase-mediated antiviral function of SAMHD1 is limited to retroviruses. In addition, neither inhibiting reverse transcription by treatment with several reverse transcriptase inhibitors nor infection with reverse transcriptase-defective HIV-1 altered RNA levels after viral challenge, indicating that the retrovirus-specific RNase function is not dependent on processes associated with retroviral reverse transcription. Conclusions: The results presented herein suggest that the RNase activity of SAMHD1 is sufficient to control the replication of retroviruses, but not that of non-retro RNA viruses. Electronic supplementary material: The online version of this article (doi:10.1186/s12977-015-0174-4) contains supplementary material, which is available to authorized users. äSAMHD1 specifically restricts retroviruses through its RNase activity (epmc).pdf DeepDyve Pubget Overpricing