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2011 ; 242
(2
): 148-56
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The effects of refractive index heterogeneity within kidney tissue on multiphoton
fluorescence excitation microscopy
#MMPMID21118239
Young PA
; Clendenon SG
; Byars JM
; Dunn KW
J Microsc
2011[May]; 242
(2
): 148-56
PMID21118239
show ga
Although multiphoton fluorescence excitation microscopy has improved the depth at
which useful fluorescence images can be collected in biological tissues, the
reach of multiphoton fluorescence excitation microscopy is nonetheless limited by
tissue scattering and spherical aberration. Scattering can be reduced in fixed
samples by mounting in a medium whose refractive index closely matches that of
the fixed material. Using optical 'clearing', the effects of refractive index
heterogeneity on signal attenuation with depth are investigated. Quantitative
measurements show that by mounting kidney tissue in a high refractive index
medium, less than 50% of signal attenuates in 100 ?m of depth.