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Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Cell+Rep 2015 ; 11 (8): 1184-92 Nephropedia Template TP
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Transmembrane complexes of DAP12 crystallized in lipid membranes provide insights into control of oligomerization in immunoreceptor assembly #MMPMID25981043
Knoblich K; Park S; Lutfi M; Hag Lv; Conn CE; Seabrook SA; Newman J; Czabotar PE; Im W; Call ME; Call MJ
Cell Rep 2015[May]; 11 (8): 1184-92 PMID25981043show ga
The membrane-spanning ?-helices of single-pass receptors play crucial roles in stabilizing oligomeric structures and transducing biochemical signals across the membrane. Probing intermolecular transmembrane interactions in single-pass receptors presents unique challenges, reflected in a gross underrepresentation of their membrane-embedded domains in structural databases. Here we present two high-resolution structures of transmembrane assemblies from a eukaryotic single-pass protein crystallized in a lipidic membrane environment. Trimeric and tetrameric structures of the immunoreceptor signaling module DAP12, determined to 1.77 Å and 2.14 Å resolution, respectively, are organized by the same polar surfaces that govern intramembrane assembly with client receptors. We demonstrate that both trimeric and tetrameric products form in cells and that formation of products larger than dimers is competitive with receptor association in the ER. The polar transmembrane sequences therefore act as primary determinants of oligomerization specificity through interplay between charge-shielding and sequestration of polar surfaces within helix interfaces.