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10.1038/srep10667 http://scihub22266oqcxt.onion/10.1038/srep10667 C4448132!4448132 !26021757     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=26021757 &cmd=llinks): Failed to open stream: HTTP request failed! 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The Cullin 4A/B-DDB1-Cereblon E3 Ubiquitin Ligase Complex Mediates the Degradation of CLC-1 Chloride Channels |pdf=|usr=}}{{26021757 }} gab.com Text The Cullin 4A/B-DDB1-Cereblon E3 Ubiquitin Ligase Complex Mediates the Degradation of CLC-1 Chloride Channels JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26021757 #FOAvip Voltage-gated CLC-1 chloride channels play a critical role in controlling the membrane excitability of skeletal muscles. Mutations in human CLC-1 channels have been linked to the hereditary muscle disorder myotonia congenita. We have previously demonstrated that disease-associated CLC-1 A531V mutant protein may fail to pass the endoplasmic reticulum quality control system and display enhanced protein degradation as well as defective membrane trafficking. Currently the molecular basis of protein degradation for CLC-1 channels is virtually unknown. Here we aim to identify the E3 ubiquitin ligase of CLC-1 channels. The protein abundance of CLC-1 was notably enhanced in the presence of MLN4924, a specific inhibitor of cullin-RING E3 ligases. Subsequent investigation with dominant-negative constructs against specific subtypes of cullin-RING E3 ligases suggested that CLC-1 seemed to serve as the substrate for cullin 4A (CUL4A) and 4B (CUL4B). Biochemical examinations further indicated that CUL4A/B, damage-specific DNA binding protein 1 (DDB1), and cereblon (CRBN) appeared to co-exist in the same protein complex with CLC-1. Moreover, suppression of CUL4A/B E3 ligase activity significantly enhanced the functional expression of the A531V mutant. Our data are consistent with the idea that the CUL4A/B-DDB1-CRBN complex catalyses the polyubiquitination and thus controls the degradation of CLC-1 channels. Twit Text FOAVip The Cullin 4A/B-DDB1-Cereblon E3 Ubiquitin Ligase Complex Mediates the Degradation of CLC-1 Chloride Channels ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=26021757 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=26021757 #FOAvip English Wikipedia <ref>{{Cite pmid|26021757 }}</ref> The Cullin 4A/B-DDB1-Cereblon E3 Ubiquitin Ligase Complex Mediates the Degradation of CLC-1 Chloride Channels #MMPMID26021757 Chen YA ; Peng YJ ; Hu MC ; Huang JJ ; Chien YC ; Wu JT ; Chen TY ; Tang CY Sci Rep 2015[May]; 5 (ä): 10667 PMID26021757 show ga Voltage-gated CLC-1 chloride channels play a critical role in controlling the membrane excitability of skeletal muscles. Mutations in human CLC-1 channels have been linked to the hereditary muscle disorder myotonia congenita. We have previously demonstrated that disease-associated CLC-1 A531V mutant protein may fail to pass the endoplasmic reticulum quality control system and display enhanced protein degradation as well as defective membrane trafficking. Currently the molecular basis of protein degradation for CLC-1 channels is virtually unknown. Here we aim to identify the E3 ubiquitin ligase of CLC-1 channels. The protein abundance of CLC-1 was notably enhanced in the presence of MLN4924, a specific inhibitor of cullin-RING E3 ligases. Subsequent investigation with dominant-negative constructs against specific subtypes of cullin-RING E3 ligases suggested that CLC-1 seemed to serve as the substrate for cullin 4A (CUL4A) and 4B (CUL4B). Biochemical examinations further indicated that CUL4A/B, damage-specific DNA binding protein 1 (DDB1), and cereblon (CRBN) appeared to co-exist in the same protein complex with CLC-1. Moreover, suppression of CUL4A/B E3 ligase activity significantly enhanced the functional expression of the A531V mutant. Our data are consistent with the idea that the CUL4A/B-DDB1-CRBN complex catalyses the polyubiquitination and thus controls the degradation of CLC-1 channels. |Adaptor Proteins, Signal Transducing [MESH] |Chloride Channels/biosynthesis/*metabolism [MESH] |Cullin Proteins/*genetics/metabolism [MESH] |DNA-Binding Proteins/*genetics/metabolism [MESH] |Humans [MESH] |Multiprotein Complexes/genetics/metabolism [MESH] |Muscle, Skeletal/metabolism/pathology [MESH] |Mutation [MESH] |Myotonia Congenita/genetics/metabolism/pathology [MESH] |Peptide Hydrolases/*genetics/metabolism [MESH] |Proteolysis [MESH]The Cullin 4A/B-DDB1-Cereblon E3 Ubiquitin Ligase Complex Mediates the(epmc).pdf DeepDyve Pubget Overpricing