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10.1002/art.39087 http://scihub22266oqcxt.onion/10.1002/art.39087 C4446227!4446227!25708025     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 278.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Arthritis+Rheumatol 2015 ; 67 (6): 1503-13 Nephropedia Template TP {{tp|p=25708025|t=2015. Lubricin/Proteoglycan 4 Binding to CD44 Receptor: A Mechanism of Lubricin?s suppression of Pro-inflammatory Cytokine Induced Synoviocyte Proliferation |pdf=|usr=}}{{25708025}} gab.com Text Lubricin/Proteoglycan 4 Binding to CD44 Receptor: A Mechanism of Lubricin s suppression of Pro-inflammatory Cytokine Induced Synoviocyte Proliferation JO: Arthritis Rheumatol http://www.kidney.de/pget.php?&tw=1&pmid=25708025 #FOAvip Objective: To evaluate recombinant human proteoglycan 4 (rhPRG4) binding to CD44 receptor and its consequence on cytokine induced synoviocyte proliferation. Methods: rhPRG4 binding to CD44 and competition with high molecular weight hyaluronic acid (HMW HA) was evaluated using a direct enzyme linked immunosorbent assay (ELISA) and surface plasmon resonance. Sialidase-A and O-glycosidase digestion of rhPRG4 was performed and CD44 binding was evaluated using ELISA. Rheumatoid arthritis fibroblast-like synoviocytes (RA-FLS) were stimulated with interleukin-1 beta (IL-1?) or tumor necrosis factor alpha (TNF-?) for 48 hours in the presence or absence of rhPRG4 or HMW HA at 20, 40 and 80?g/ml and cell proliferation was measured. CD44 contribution was assessed by co-incubation with a CD44 antibody (IM7). The anti-proliferative effect of rhPRG4 was investigated following treatment of Prg4?/? synoviocytes with IL-1? or TNF-? in the presence or absence of IM7. Results: rhPRG4 binds CD44 and interferes with HMW HA CD44 binding. Removal of sialic acid and O-glycosylations significantly increased CD44 binding by rhPRG4 (p<0.001). rhPRG4 and HMW HA at 40 and 80?g/ml significantly suppressed IL-1? induced RA-FLS proliferation (p<0.05). rhPRG4 at 20, 40 and 80?g/ml significantly suppressed TNF-? induced RA-FLS proliferation (p<0.05). CD44 neutralization reversed the effect of rhPRG4 on IL-1? and TNF-? stimulated RA-FLS and the effect of HMW HA on IL-1? stimulated RA-FLS. rhPRG4 inhibited cytokine-induced proliferation of Prg4?/? synoviocytes which could be prevented by blocking CD44. Conclusion: Lubricin is a novel putative ligand for CD44 and may control synoviocyte overgrowth in inflammatory arthropathies via a CD44-mediated mechanism. Twit Text FOAVip Lubricin/Proteoglycan 4 Binding to CD44 Receptor: A Mechanism of Lubricin s suppression of Pro-inflammatory Cytokine Induced Synoviocyte Proliferation ????Arthritis Rheumatol http://www.kidney.de/pget.php?&tw=1&pmid=25708025 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25708025 #FOAvip English Wikipedia <ref>{{Cite pmid|25708025}}</ref> Lubricin/Proteoglycan 4 Binding to CD44 Receptor: A Mechanism of Lubricin?s suppression of Pro-inflammatory Cytokine Induced Synoviocyte Proliferation #MMPMID25708025 Al-Sharif A; Jamal M; Zhang L; Larson K; Schmidt T; Jay G; Elsaid K Arthritis Rheumatol 2015[Jun]; 67 (6): 1503-13 PMID25708025show ga Objective: To evaluate recombinant human proteoglycan 4 (rhPRG4) binding to CD44 receptor and its consequence on cytokine induced synoviocyte proliferation. Methods: rhPRG4 binding to CD44 and competition with high molecular weight hyaluronic acid (HMW HA) was evaluated using a direct enzyme linked immunosorbent assay (ELISA) and surface plasmon resonance. Sialidase-A and O-glycosidase digestion of rhPRG4 was performed and CD44 binding was evaluated using ELISA. Rheumatoid arthritis fibroblast-like synoviocytes (RA-FLS) were stimulated with interleukin-1 beta (IL-1?) or tumor necrosis factor alpha (TNF-?) for 48 hours in the presence or absence of rhPRG4 or HMW HA at 20, 40 and 80?g/ml and cell proliferation was measured. CD44 contribution was assessed by co-incubation with a CD44 antibody (IM7). The anti-proliferative effect of rhPRG4 was investigated following treatment of Prg4?/? synoviocytes with IL-1? or TNF-? in the presence or absence of IM7. Results: rhPRG4 binds CD44 and interferes with HMW HA CD44 binding. Removal of sialic acid and O-glycosylations significantly increased CD44 binding by rhPRG4 (p<0.001). rhPRG4 and HMW HA at 40 and 80?g/ml significantly suppressed IL-1? induced RA-FLS proliferation (p<0.05). rhPRG4 at 20, 40 and 80?g/ml significantly suppressed TNF-? induced RA-FLS proliferation (p<0.05). CD44 neutralization reversed the effect of rhPRG4 on IL-1? and TNF-? stimulated RA-FLS and the effect of HMW HA on IL-1? stimulated RA-FLS. rhPRG4 inhibited cytokine-induced proliferation of Prg4?/? synoviocytes which could be prevented by blocking CD44. Conclusion: Lubricin is a novel putative ligand for CD44 and may control synoviocyte overgrowth in inflammatory arthropathies via a CD44-mediated mechanism. äLubricin/Proteoglycan 4 Binding to CD44 Receptor: A Mechanism of Lubri(epmc).pdf DeepDyve Pubget Overpricing