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Screening and identification of the differential proteins in kidney with complete
unilateral ureteral obstruction
#MMPMID26045767
Zhao Q
; Yang Y
; Wang CL
; Hou Y
; Chen H
Int J Clin Exp Pathol
2015[]; 8
(3
): 2615-26
PMID26045767
show ga
Obstructive nephropathy is a major cause of renal failure, particularly in
infants and children, and indications for therapeutic intervention remain highly
controversial. There is a great need for the development of new methods to
monitor patients, and the biomarker research field is a promising approach for
this purpose to be used as prognostic tools for early disease detection and the
choice of the optimal treatment and monitoring. Here, we presented our
comparative proteomics study of rat kidney with complete unilateral ureteral
obstruction (CUUO). Proteins from the groups of CUUO and corresponding sham rat
kidney tissues were subjected to 2-D gel electrophoresis, and then protein
identification by mass spectrometry. We identified 39 proteins with differential
expression between kidney tissues from sham operated group and those with CUUO.
These identified proteins were reported to be involved in cell apoptosis, energy
metabolism and injuries of mitochondrion and oxidative stress, and so on. We
confirmed 3 identified proteins by immunoblot analysis and immunofluorescence
staining and assessed their mRNA levels in renal tissues. Our results demonstrate
protein alterations that reflect the pathological situation of the obstructed
kidneys, which may help understand the relationship between oxidative stress and
obstructive nephropathy.
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