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10.5114/ceji.2014.45937

http://scihub22266oqcxt.onion/10.5114/ceji.2014.45937
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suck abstract from ncbi


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pmid26155136      Cent+Eur+J+Immunol 2014 ; 39 (3): 279-84
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  • Xenotransplantation of human cultured parathyroid progenitor cells into mouse peritoneum does not induce rejection reaction #MMPMID26155136
  • Nawrot I; Wo?niewicz B; Szmidt J; ?ladowski D; Zaj?c K; Chudzi?ski W
  • Cent Eur J Immunol 2014[]; 39 (3): 279-84 PMID26155136show ga
  • Introduction: Parathyroid progenitor cells devoid of immunogenic antigens were used for human allotransplantation. Although there were many potential reasons for the expiry of transplant activity in humans, we decided to exclude a subclinical form of rejection reaction, and test the rejection reaction in an animal model. Material and methods: Experiments were carried out on 40 conventional male mice in their third month of life. The animals were housed in groups of 10 per cage in 4 cages with fitted water dispensers and fed a conventional diet based on standard pellet food. They were divided into four groups of 10 animals each, three experimental groups and one control group. Identified progenitor cells were stored in a cell bank. After testing the phenotype, viability, and absence of immunogenic properties, the cells were transplanted into mouse peritoneum cavity. Results: Animals were observed for 9 weeks. At 9 weeks of observation, the mean serum PTH concentration in the experimental groups was 2.0-2.5 pg/ml, while in the control group it did not exceed 1.5 pg/ml. The immunohistochemical assays demonstrated that millions of viable cells with a phenotype identical to the endocrine cells had survived in the peritoneum. Histologic specimens from different internal organs stained for PTH revealed positive cells labelled with anti-PTH Ab in the intestinal lamina, brain, liver, and spleen. Conclusions: In the present paper we have demonstrated that xenotransplantation may be used as a model for an explanation of the immunogenic properties of cells generated from postnatal organs for regenerative therapy.
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