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Xenotransplantation of human cultured parathyroid progenitor cells into mouse
peritoneum does not induce rejection reaction
#MMPMID26155136
Nawrot I
; Wo?niewicz B
; Szmidt J
; ?ladowski D
; Zaj?c K
; Chudzi?ski W
Cent Eur J Immunol
2014[]; 39
(3
): 279-84
PMID26155136
show ga
INTRODUCTION: Parathyroid progenitor cells devoid of immunogenic antigens were
used for human allotransplantation. Although there were many potential reasons
for the expiry of transplant activity in humans, we decided to exclude a
subclinical form of rejection reaction, and test the rejection reaction in an
animal model. MATERIAL AND METHODS: Experiments were carried out on 40
conventional male mice in their third month of life. The animals were housed in
groups of 10 per cage in 4 cages with fitted water dispensers and fed a
conventional diet based on standard pellet food. They were divided into four
groups of 10 animals each, three experimental groups and one control group.
Identified progenitor cells were stored in a cell bank. After testing the
phenotype, viability, and absence of immunogenic properties, the cells were
transplanted into mouse peritoneum cavity. RESULTS: Animals were observed for 9
weeks. At 9 weeks of observation, the mean serum PTH concentration in the
experimental groups was 2.0-2.5 pg/ml, while in the control group it did not
exceed 1.5 pg/ml. The immunohistochemical assays demonstrated that millions of
viable cells with a phenotype identical to the endocrine cells had survived in
the peritoneum. Histologic specimens from different internal organs stained for
PTH revealed positive cells labelled with anti-PTH Ab in the intestinal lamina,
brain, liver, and spleen. CONCLUSIONS: In the present paper we have demonstrated
that xenotransplantation may be used as a model for an explanation of the
immunogenic properties of cells generated from postnatal organs for regenerative
therapy.
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