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2015 ; 66
(5
): 1157-63
Nephropedia Template TP
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English Wikipedia
Lipotubuloids in ovary epidermis of Ornithogalum umbellatum act as metabolons:
suggestion of the name lipotubuloid metabolon
#MMPMID25540439
Kwiatkowska M
; Polit JT
; St?pi?ski D
; Pop?o?ska K
; Wojtczak A
; Dom?nguez E
; Heredia A
J Exp Bot
2015[Mar]; 66
(5
): 1157-63
PMID25540439
show ga
A metabolon is a temporary, structural-functional complex formed between
sequential metabolic enzymes and cellular elements. Cytoplasmic domains called
lipotubuloids are present in Ornithogalum umbellatum ovary epidermis. They
consist of numerous lipid bodies entwined with microtubules, polysomes, rough
endoplasmic reticulum (RER), and actin filaments connected to microtubules
through myosin and kinesin. A few mitochondria, Golgi structures, and microbodies
are also observed and also, at later development stages, autolytic vacuoles. Each
lipotubuloid is surrounded by a tonoplast as it invaginates into a vacuole. These
structures appear in young cells, which grow intensively reaching 30-fold
enlargement but do not divide. They also become larger due to an increasing
number of lipid bodies formed in the RER by the accumulation of lipids between
leaflets of the phospholipid bilayer. When a cell ceases to grow, the
lipotubuloids disintegrate into individual structures. Light and electron
microscope studies using filming techniques, autoradiography with [(3)H]palmitic
acid, immunogold labelling with antibodies against DGAT2, phospholipase D1 and
lipase, and double immunogold labelling with antibodies against myosin and
kinesin, as well as experiments with propyzamide, a microtubule activity
inhibitor, have shown that lipotubuloids are functionally and structurally
integrated metabolons [here termed lipotubuloid metabolons (LMs)] occurring
temporarily in growing cells. They synthesize lipids in lipid bodies in
cooperation with microtubules. Some of these lipids are metabolized and used by
the cell as nutrients, and others are transformed into cuticle whose formation is
mediated by cutinsomes. The latter were discovered in planta using specific
anti-cutinsome antibodies visualized by gold labelling. Moreover, LMs are able to
rotate autonomously due to the interaction of microtubules, actin filaments, and
motor proteins, which influence microtubules by changing their diameter.