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.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117 J+Immunol+Res
2015 ; 2015
(ä): 983094
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Increased Performances of the Biological Diagnosis of the Antiphospholipid
Syndrome by the Use of a Multiplex Assay
#MMPMID26065010
Sénant M
; Rostane H
; Fernani-Oukil F
; Hosking F
; Bellery F
; Courchinoux A
; Tartour E
; Darnige L
; Dragon-Durey MA
J Immunol Res
2015[]; 2015
(ä): 983094
PMID26065010
show ga
Antiphospholipid syndrome (APS) is characterized by development of venous and/or
arterial thrombosis and pregnancy morbidity. Biological criteria are the
persistent presence of lupus anticoagulant (LA) and/or anti-cardiolipin (aCL)
and/or anti-B2GP1 autoantibodies' positivity. The assays' performances are of
crucial importance. We evaluated a multiplex assay allowing simultaneous
detection of IgG anti-cardiolipin, anti-B2GP1, and anti-factor II. 300 samples
were tested. Patients were categorized according to clinical scores of APS from 0
to 3 based on presence or not of arterial or venous thrombosis, fetal loss, and
autoimmunity. We used a multiplex assay for APS for simultaneous detection of
aCL, anti-B2GP1, and factor II and compared its performances to ELISA assays.
Presence of LA was also assessed. We performed a correlation study of the tested
assays and compared their clinical efficacy by ROC curve analysis. We obtained
significantly higher performances with the multiplex assay than ELISA with higher
area under the curve (AUC). The disease rate increased with the number of
positive markers from 9% for 1 marker to 100% for 4 markers positive for patients
with high risk scores. The multiplex APS assay exhibited higher performances
particularly in case of primary APS and is useful for rapid diagnosis of APS.