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10.1016/j.transproceed.2014.05.075 http://scihub22266oqcxt.onion/10.1016/j.transproceed.2014.05.075 C4435942!4435942!25131084     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 300.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 300.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 300.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Transplant+Proc 2014 ; 46 (6): 1967-71 Nephropedia Template TP {{tp|p=25131084|t=2014. Improved Coating of Pancreatic Islets with Regulatory T cells (Tregs) to Create Local Immunosuppression by Using Biotin-Polyethylene glycol-Succinimidyl Valeric Acid Ester Molecule |pdf=|usr=}}{{25131084}} gab.com Text Improved Coating of Pancreatic Islets with Regulatory T cells (Tregs) to Create Local Immunosuppression by Using Biotin-Polyethylene glycol-Succinimidyl Valeric Acid Ester Molecule JO: Transplant Proc http://www.kidney.de/pget.php?&tw=1&pmid=25131084 #FOAvip Background: We showed that T regulatory (Treg) cells can be attached to the surface of pancreatic islets providing local immunoprotection. Further optimization of the method can improve coating efficiency, which may prolong graft survival. In this study, we compared the effectiveness of two different molecules used for binding of the Tregs to the surface of pancreatic islets. Our aim was to increase the number of Treg cells attached to islets without compromising islets viability and function. Methods: Cell surface of human Treg cells and pancreatic islets was modified using biotin-polyethylene glycol (-PEG-) -N-hydroxylsuccinimide (biotin-PEG-NHS) or -succinimidyl valeric acid ester (biotin-PEG-SVA). Then, islets were incubated with streptavidin as islet/Treg cells binding molecule. Treg cells were stained with CellTracker? CM-DiL dye and visualized using Laser Scanning Confocal Microscope. The number of Treg cells attached per islets surface area was analyzed by Imaris software. The effect of coating on islet functionality was determined using Glucose-Stimulated Insulin Response (GSIR) assay. Results: Coating procedure with biotin-PEG-SVA allowed for attaching 40% more Treg cells per 1 ?m2 of islets surface. While viability was comparable, function of the islets after coating using biotin-PEG-SVA molecule was better preserved than with NHS molecule. GSIR was 62% higher for islets coated with biotin-PEG-SVA compared to biotin-PEG-NHS. Conclusion: Coating of islets with Treg cells using biotin-PEG-SVA improves effectiveness with better preservation of the islet function. Improvement of the method of coating pancreatic islets with Treg cells, could further facilitate the effectiveness of this novel immunoprotective approach and translation into clinical settings. Twit Text FOAVip Improved Coating of Pancreatic Islets with Regulatory T cells (Tregs) to Create Local Immunosuppression by Using Biotin-Polyethylene glycol-Succinimidyl Valeric Acid Ester Molecule ????Transplant Proc http://www.kidney.de/pget.php?&tw=1&pmid=25131084 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25131084 #FOAvip English Wikipedia <ref>{{Cite pmid|25131084}}</ref> Improved Coating of Pancreatic Islets with Regulatory T cells (Tregs) to Create Local Immunosuppression by Using Biotin-Polyethylene glycol-Succinimidyl Valeric Acid Ester Molecule #MMPMID25131084 Go??b K; Kizilel S; Bal T; Hara M; Zielinski M; Grose R; Savari O; Wang XJ; Wang LJ; Tibudan M; Krzystyniak A; Marek-Trzonkowska N; Millis JM; Trzonkowski P; Witkowski P Transplant Proc 2014[Jul]; 46 (6): 1967-71 PMID25131084show ga Background: We showed that T regulatory (Treg) cells can be attached to the surface of pancreatic islets providing local immunoprotection. Further optimization of the method can improve coating efficiency, which may prolong graft survival. In this study, we compared the effectiveness of two different molecules used for binding of the Tregs to the surface of pancreatic islets. Our aim was to increase the number of Treg cells attached to islets without compromising islets viability and function. Methods: Cell surface of human Treg cells and pancreatic islets was modified using biotin-polyethylene glycol (-PEG-) -N-hydroxylsuccinimide (biotin-PEG-NHS) or -succinimidyl valeric acid ester (biotin-PEG-SVA). Then, islets were incubated with streptavidin as islet/Treg cells binding molecule. Treg cells were stained with CellTracker? CM-DiL dye and visualized using Laser Scanning Confocal Microscope. The number of Treg cells attached per islets surface area was analyzed by Imaris software. The effect of coating on islet functionality was determined using Glucose-Stimulated Insulin Response (GSIR) assay. Results: Coating procedure with biotin-PEG-SVA allowed for attaching 40% more Treg cells per 1 ?m2 of islets surface. While viability was comparable, function of the islets after coating using biotin-PEG-SVA molecule was better preserved than with NHS molecule. GSIR was 62% higher for islets coated with biotin-PEG-SVA compared to biotin-PEG-NHS. Conclusion: Coating of islets with Treg cells using biotin-PEG-SVA improves effectiveness with better preservation of the islet function. Improvement of the method of coating pancreatic islets with Treg cells, could further facilitate the effectiveness of this novel immunoprotective approach and translation into clinical settings. äImproved Coating of Pancreatic Islets with Regulatory T cells (Tregs) (epmc).pdf DeepDyve Pubget Overpricing