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10.1016/j.fgb.2014.11.001 http://scihub22266oqcxt.onion/10.1016/j.fgb.2014.11.001 C4433448!4433448!25460849     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Fungal+Genet+Biol 2015 ; 78 (ä): 99-107 Nephropedia Template TP {{tp|p=25460849|t=2015. New technology and resources for cryptococcal research |pdf=|usr=}}{{25460849}} gab.com Text New technology and resources for cryptococcal research JO: Fungal Genet Biol http://www.kidney.de/pget.php?&tw=1&pmid=25460849 #FOAvip Rapid advances in molecular biology and genome sequencing have enabled the generation of new technology and resources for cryptococcal research. RNAi-mediated specific gene knock down has become routine and more efficient by utilizing modified shRNA plasmids and convergent promoter RNAi constructs. This system was recently applied in a high-throughput screen to identify genes involved in host-pathogen interactions. Gene deletion efficiencies have also been improved by increasing rates of homologous recombination through a number of approaches, including a combination of double-joint PCR with split-marker transformation, the use of dominant selectable markers and the introduction of Cre-Loxp systems into Cryptococcus. Moreover, visualization of cryptococcal proteins has become more facile using fusions with codon-optimized fluorescent tags, such as green or red fluorescent proteins or, mCherry. Using recent genome-wide analytical tools, new transcriptional factors and regulatory proteins have been identified in novel virulence-related signaling pathways by employing microarray analysis, RNA-sequencing and proteomic analysis. Twit Text FOAVip New technology and resources for cryptococcal research ????Fungal Genet Biol http://www.kidney.de/pget.php?&tw=1&pmid=25460849 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25460849 #FOAvip English Wikipedia <ref>{{Cite pmid|25460849}}</ref> New technology and resources for cryptococcal research #MMPMID25460849 Zhang N; Park YD; Williamson PR Fungal Genet Biol 2015[May]; 78 (ä): 99-107 PMID25460849show ga Rapid advances in molecular biology and genome sequencing have enabled the generation of new technology and resources for cryptococcal research. RNAi-mediated specific gene knock down has become routine and more efficient by utilizing modified shRNA plasmids and convergent promoter RNAi constructs. This system was recently applied in a high-throughput screen to identify genes involved in host-pathogen interactions. Gene deletion efficiencies have also been improved by increasing rates of homologous recombination through a number of approaches, including a combination of double-joint PCR with split-marker transformation, the use of dominant selectable markers and the introduction of Cre-Loxp systems into Cryptococcus. Moreover, visualization of cryptococcal proteins has become more facile using fusions with codon-optimized fluorescent tags, such as green or red fluorescent proteins or, mCherry. Using recent genome-wide analytical tools, new transcriptional factors and regulatory proteins have been identified in novel virulence-related signaling pathways by employing microarray analysis, RNA-sequencing and proteomic analysis. äNew technology and resources for cryptococcal research (epmc).pdf DeepDyve Pubget Overpricing