Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1016/j.molcel.2015.03.017 http://scihub22266oqcxt.onion/10.1016/j.molcel.2015.03.017 C4427541!4427541 !25921072     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\25921072 .jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Mol+Cell 2015 ; 58 (3 ): 483-94 Nephropedia Template TP {{tp|p=25921072 |t=2015. The dynamics of eukaryotic replication initiation: origin specificity, licensing, and firing at the single-molecule level |pdf=|usr=}}{{25921072 }} gab.com Text The dynamics of eukaryotic replication initiation: origin specificity, licensing, and firing at the single-molecule level JO: Mol Cell http://www.kidney.de/pget.php?&tw=1&pmid=25921072 #FOAvip Eukaryotic replication initiation is highly regulated and dynamic. It begins with the origin recognition complex (ORC) binding DNA sites called origins of replication. ORC, together with Cdc6 and Cdt1, mediate pre-replicative complex (pre-RC) assembly by loading a double hexamer of Mcm2-7: the core of the replicative helicase. Here, we use single-molecule imaging to directly visualize Saccharomyces cerevisiae pre-RC assembly and replisome firing in real time. We show that ORC can locate and stably bind origins within large tracts of non-origin DNA and that Cdc6 drives ordered pre-RC assembly. We further show that the dynamics of the ORC-Cdc6 interaction dictate Mcm2-7 loading specificity and that Mcm2-7 double hexamers form preferentially at a native origin sequence. Finally, we demonstrate that single Mcm2-7 hexamers propagate bidirectionally, monotonically, and processively as constituents of active replisomes. Twit Text FOAVip The dynamics of eukaryotic replication initiation: origin specificity, licensing, and firing at the single-molecule level ????Mol Cell http://www.kidney.de/pget.php?&tw=1&pmid=25921072 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25921072 #FOAvip English Wikipedia <ref>{{Cite pmid|25921072 }}</ref> The dynamics of eukaryotic replication initiation: origin specificity, licensing, and firing at the single-molecule level #MMPMID25921072 Duzdevich D ; Warner MD ; Ticau S ; Ivica NA ; Bell SP ; Greene EC Mol Cell 2015[May]; 58 (3 ): 483-94 PMID25921072 show ga Eukaryotic replication initiation is highly regulated and dynamic. It begins with the origin recognition complex (ORC) binding DNA sites called origins of replication. ORC, together with Cdc6 and Cdt1, mediate pre-replicative complex (pre-RC) assembly by loading a double hexamer of Mcm2-7: the core of the replicative helicase. Here, we use single-molecule imaging to directly visualize Saccharomyces cerevisiae pre-RC assembly and replisome firing in real time. We show that ORC can locate and stably bind origins within large tracts of non-origin DNA and that Cdc6 drives ordered pre-RC assembly. We further show that the dynamics of the ORC-Cdc6 interaction dictate Mcm2-7 loading specificity and that Mcm2-7 double hexamers form preferentially at a native origin sequence. Finally, we demonstrate that single Mcm2-7 hexamers propagate bidirectionally, monotonically, and processively as constituents of active replisomes. |Algorithms [MESH] |Binding Sites/genetics [MESH] |Cell Cycle Proteins/genetics/metabolism [MESH] |DNA Replication/*genetics [MESH] |DNA, Fungal/genetics/metabolism [MESH] |DNA-Binding Proteins/genetics/metabolism [MESH] |Eukaryotic Cells/metabolism [MESH] |Kinetics [MESH] |Minichromosome Maintenance Proteins/genetics/metabolism [MESH] |Models, Genetic [MESH] |Origin Recognition Complex/*genetics/metabolism [MESH] |Protein Binding [MESH] |Replication Origin/genetics [MESH] |Saccharomyces cerevisiae Proteins/*genetics/metabolism [MESH] |Saccharomyces cerevisiae/*genetics/metabolism [MESH]The dynamics of eukaryotic replication initiation: origin specificity,(epmc).pdf DeepDyve Pubget Overpricing