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10.1074/jbc.M115.636969 http://scihub22266oqcxt.onion/10.1074/jbc.M115.636969 C4424337!4424337 !25809486     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25809486 &cmd=llinks): Failed to open stream: HTTP request failed! 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Dynamic Sialylation in Transforming Growth Factor-? (TGF-?)-induced Epithelial to Mesenchymal Transition |pdf=|usr=}}{{25809486 }} gab.com Text Dynamic Sialylation in Transforming Growth Factor- (TGF- )-induced Epithelial to Mesenchymal Transition JO: J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=25809486 #FOAvip Epithelial-mesenchymal transition (EMT) is a fundamental process in embryonic development and organ formation. Aberrant regulation of EMT often leads to tumor progression. Changes in cell surface sialylation have recently been implicated in mediating EMT. Herein we report the visualization of dynamic changes of sialylation and glycoproteomic analysis of newly synthesized sialylated proteins in EMT by metabolic labeling of sialylated glycans with azides, followed by click labeling with fluorophores or affinity tags. We discovered that sialylation was down-regulated during EMT but then reverted and up-regulated in the mesenchymal state after EMT, accompanied by mRNA expression level changes of genes involved in the sialic acid biosynthesis. Quantitative proteomic analysis identified a list of sialylated proteins whose biosynthesis was dynamically regulated during EMT. Sialylation of cell surface adherent receptor integrin ?4 was found to be down-regulated, which may regulate integrin functions during EMT. Furthermore, a global sialylation inhibitor was used to probe the functional role of sialylation during EMT. We found that inhibition of sialylation promoted EMT. Taken together, our findings suggest the important role of sialylation in regulating EMT and imply its possible function in related pathophysiological events, such as cancer metastasis. Twit Text FOAVip Dynamic Sialylation in Transforming Growth Factor- (TGF- )-induced Epithelial to Mesenchymal Transition ????J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=25809486 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25809486 #FOAvip English Wikipedia <ref>{{Cite pmid|25809486 }}</ref> Dynamic Sialylation in Transforming Growth Factor-? (TGF-?)-induced Epithelial to Mesenchymal Transition #MMPMID25809486 Du J ; Hong S ; Dong L ; Cheng B ; Lin L ; Zhao B ; Chen YG ; Chen X J Biol Chem 2015[May]; 290 (19 ): 12000-13 PMID25809486 show ga Epithelial-mesenchymal transition (EMT) is a fundamental process in embryonic development and organ formation. Aberrant regulation of EMT often leads to tumor progression. Changes in cell surface sialylation have recently been implicated in mediating EMT. Herein we report the visualization of dynamic changes of sialylation and glycoproteomic analysis of newly synthesized sialylated proteins in EMT by metabolic labeling of sialylated glycans with azides, followed by click labeling with fluorophores or affinity tags. We discovered that sialylation was down-regulated during EMT but then reverted and up-regulated in the mesenchymal state after EMT, accompanied by mRNA expression level changes of genes involved in the sialic acid biosynthesis. Quantitative proteomic analysis identified a list of sialylated proteins whose biosynthesis was dynamically regulated during EMT. Sialylation of cell surface adherent receptor integrin ?4 was found to be down-regulated, which may regulate integrin functions during EMT. Furthermore, a global sialylation inhibitor was used to probe the functional role of sialylation during EMT. We found that inhibition of sialylation promoted EMT. Taken together, our findings suggest the important role of sialylation in regulating EMT and imply its possible function in related pathophysiological events, such as cancer metastasis. |*Epithelial-Mesenchymal Transition [MESH] |*Gene Expression Regulation [MESH] |Cell Adhesion [MESH] |Cell Line, Tumor [MESH] |Cell Membrane/*metabolism [MESH] |Cell Movement [MESH] |Cytidine Monophosphate N-Acetylneuraminic Acid/chemistry [MESH] |Down-Regulation [MESH] |Glycosylation [MESH] |Humans [MESH] |Integrin beta4/metabolism [MESH] |Microscopy, Confocal [MESH] |N-Acetylneuraminic Acid/*chemistry [MESH] |Neoplasm Metastasis [MESH] |Proteomics [MESH] |RNA, Messenger/metabolism [MESH]Dynamic Sialylation in Transforming Growth Factor-? (TGF-?)-induced Ep(epmc).pdf DeepDyve Pubget Overpricing