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10.4049/jimmunol.1402979 http://scihub22266oqcxt.onion/10.4049/jimmunol.1402979 C4417646!4417646!25840911     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Immunol 2015 ; 194 (10): 4705-16 Nephropedia Template TP {{tp|p=25840911|t=2015. Macrophage epoxygenase determines a pro-fibrotic transcriptome signature |pdf=|usr=}}{{25840911}} gab.com Text Macrophage epoxygenase determines a pro-fibrotic transcriptome signature JO: J Immunol http://www.kidney.de/pget.php?&tw=1&pmid=25840911 #FOAvip Epoxygenases belong to the cytochrome P450 family and they generate epoxyeicosatrienoic acids (EETs) known to have anti-inflammatory effects but little is known about their role in macrophage function. By high-throughput sequencing of RNA (RNA-seq) in primary macrophages derived from rodents and humans, we establish the relative expression of epoxygenases in these cells. Zinc-finger nuclease-mediated targeted gene deletion of the major rat macrophage epoxygenase Cyp2j4 (orthologue of human CYP2J2), resulted in reduced EET synthesis. Cyp2j4?/? macrophages have relatively increased PPAR? levels and show a pro-fibrotic transcriptome, displaying over-expression of a specific subset of genes (260 transcripts) primarily involved in extracellular matrix, with fibronectin being the most abundantly expressed transcript. Fibronectin expression is under the control of epoxygenase activity in human and rat primary macrophages. In keeping with the in vitro findings, Cyp2j4?/? rats show up-regulation of type I collagen following unilateral ureter obstruction (UUO) of the kidney and quantitative proteomics analysis (LC-MS/MS) showed increased renal type I collagen and fibronectin protein abundance resulting from experimentally induced crescentic glomerulonephritis in these rats. Taken together, these results identify the rat epoxygenase Cyp2j4 as a determinant of a pro-fibrotic macrophage transcriptome that could have implications in various inflammatory conditions depending on macrophage function. Twit Text FOAVip Macrophage epoxygenase determines a pro-fibrotic transcriptome signature ????J Immunol http://www.kidney.de/pget.php?&tw=1&pmid=25840911 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25840911 #FOAvip English Wikipedia <ref>{{Cite pmid|25840911}}</ref> Macrophage epoxygenase determines a pro-fibrotic transcriptome signature #MMPMID25840911 Behmoaras J; Diaz AG; Venda L; Ko JH; Srivastava P; Montoya A; Faull P; Webster Z; Moyon B; Pusey CD; Abraham DJ; Petretto E; Cook TH; Aitman TJ J Immunol 2015[May]; 194 (10): 4705-16 PMID25840911show ga Epoxygenases belong to the cytochrome P450 family and they generate epoxyeicosatrienoic acids (EETs) known to have anti-inflammatory effects but little is known about their role in macrophage function. By high-throughput sequencing of RNA (RNA-seq) in primary macrophages derived from rodents and humans, we establish the relative expression of epoxygenases in these cells. Zinc-finger nuclease-mediated targeted gene deletion of the major rat macrophage epoxygenase Cyp2j4 (orthologue of human CYP2J2), resulted in reduced EET synthesis. Cyp2j4?/? macrophages have relatively increased PPAR? levels and show a pro-fibrotic transcriptome, displaying over-expression of a specific subset of genes (260 transcripts) primarily involved in extracellular matrix, with fibronectin being the most abundantly expressed transcript. Fibronectin expression is under the control of epoxygenase activity in human and rat primary macrophages. In keeping with the in vitro findings, Cyp2j4?/? rats show up-regulation of type I collagen following unilateral ureter obstruction (UUO) of the kidney and quantitative proteomics analysis (LC-MS/MS) showed increased renal type I collagen and fibronectin protein abundance resulting from experimentally induced crescentic glomerulonephritis in these rats. Taken together, these results identify the rat epoxygenase Cyp2j4 as a determinant of a pro-fibrotic macrophage transcriptome that could have implications in various inflammatory conditions depending on macrophage function. äMacrophage epoxygenase determines a pro-fibrotic transcriptome signatu(epmc).pdf DeepDyve Pubget Overpricing