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10.1016/j.jmb.2015.03.005 http://scihub22266oqcxt.onion/10.1016/j.jmb.2015.03.005 C4417415!4417415 !25772494     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=25772494 &cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       J+Mol+Biol 2015 ; 427 (11 ): 2039-2055 Nephropedia Template TP {{tp|p=25772494 |t=2015. Bimolecular Fluorescence Complementation (BiFC) Analysis: Advances and Recent Applications for Genome-Wide Interaction Studies |pdf=|usr=}}{{25772494 }} gab.com Text Bimolecular Fluorescence Complementation (BiFC) Analysis: Advances and Recent Applications for Genome-Wide Interaction Studies JO: J Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=25772494 #FOAvip Complex protein networks are involved in nearly all cellular processes. To uncover these vast networks of protein interactions, various high-throughput screening technologies have been developed. Over the last decade, bimolecular fluorescence complementation (BiFC) assay has been widely used to detect protein-protein interactions (PPIs) in living cells. This technique is based on the reconstitution of a fluorescent protein in vivo. Easy quantification of the BiFC signals allows effective cell-based high-throughput screenings for protein binding partners and drugs that modulate PPIs. Recently, with the development of large screening libraries, BiFC has been effectively applied for genome-wide PPI studies and has uncovered novel protein interactions, providing new insight into protein functions. In this review, we describe the development of reagents and methods used for BiFC-based screens in yeast, plants, and mammalian cells. We also discuss the advantages and drawbacks of these methods and highlight the application of BiFC in large-scale studies. Twit Text FOAVip Bimolecular Fluorescence Complementation (BiFC) Analysis: Advances and Recent Applications for Genome-Wide Interaction Studies ????J Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=25772494 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=25772494 #FOAvip English Wikipedia <ref>{{Cite pmid|25772494 }}</ref> Bimolecular Fluorescence Complementation (BiFC) Analysis: Advances and Recent Applications for Genome-Wide Interaction Studies #MMPMID25772494 Miller KE ; Kim Y ; Huh WK ; Park HO J Mol Biol 2015[Jun]; 427 (11 ): 2039-2055 PMID25772494 show ga Complex protein networks are involved in nearly all cellular processes. To uncover these vast networks of protein interactions, various high-throughput screening technologies have been developed. Over the last decade, bimolecular fluorescence complementation (BiFC) assay has been widely used to detect protein-protein interactions (PPIs) in living cells. This technique is based on the reconstitution of a fluorescent protein in vivo. Easy quantification of the BiFC signals allows effective cell-based high-throughput screenings for protein binding partners and drugs that modulate PPIs. Recently, with the development of large screening libraries, BiFC has been effectively applied for genome-wide PPI studies and has uncovered novel protein interactions, providing new insight into protein functions. In this review, we describe the development of reagents and methods used for BiFC-based screens in yeast, plants, and mammalian cells. We also discuss the advantages and drawbacks of these methods and highlight the application of BiFC in large-scale studies. |*Molecular Probe Techniques [MESH] |Animals [MESH] |Arabidopsis Proteins/metabolism [MESH] |Fluorescent Dyes/metabolism [MESH] |Genome-Wide Association Study [MESH] |Green Fluorescent Proteins/metabolism [MESH] |High-Throughput Screening Assays/*methods [MESH] |Humans [MESH] |Mammals [MESH] |Protein Interaction Mapping/*methods [MESH] |Proteomics/methods [MESH] |Telomere/metabolism [MESH]Bimolecular Fluorescence Complementation (BiFC) Analysis: Advances and(epmc).pdf DeepDyve Pubget Overpricing